沙棘果多糖的理化特征及其体外抗氧化活性

赵志强; 朱叙丞; 冯真颖; 陈海婷; 余佳乐; 闫笛; 宋松泉; 申迎宾; 唐翠芳

doi:10.13386/j.issn1002-0306.2022070288

沙棘果多糖的理化特征及其体外抗氧化活性

doi: 10.13386/j.issn1002-0306.2022070288

1.
广州大学生命科学学院，广东广州 510006
2.
湘南学院南岭现代种业研究院，湖南郴州 423043

基金项目: 广东省科技厅2020年省乡村振兴战略专项资金-食品中功能性成分的活性评价及系列产品开发（620096-501）。

详细信息

作者简介:
赵志强（2000−），男，本科，研究方向：食品营养，E-mail：1767750623@qq.com

通讯作者:
申迎宾（1981−），男，博士，副教授，研究方向：食品营养，E-mail：shenybin412@gmail.com

唐翠芳（1987−），女，硕士，高级物流师，研究方向：种子资源的收集、保存与开发，E-mail：yuenan2008@126.com

中图分类号: TS201.2
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- 文章访问数: 35
- HTML全文浏览量: 31
- PDF下载量: 7
- 被引次数: 0
出版历程
- 收稿日期: 2022-08-01
- 网络出版日期: 2023-05-20
- 刊出日期: 2023-07-01

Physicochemical Characteristic and Antioxidant Activity in Vitro of Seabuckthorn Fruit Polysaccharide

1.
School of Life Sciences, Guangzhou University, Guangzhou 510006, China
2.
Nanling Research Institute for Modern Seed Industry, Xiangnan Uiversity, Chenzhou 423043, China

摘要

摘要: 本研究通过热水提取法从沙棘果实中提取并初步纯化得到一种精制多糖（Seabuckthorn Berry Polysaccharide，SBP），对其单糖组成、分子量组成、微观表征、热稳定性、流变学特性等理化特征进行表征，并采用四种体外模型评价其抗氧化活性。结果表明：该多糖的主要组分是一种大分子的酸性多糖，分子量为37.82×10⁴ Da，由岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖、葡萄糖醛酸等单糖构成，其摩尔比为0.244:0.098:0.265:0.075:0.091:0.081:0.103，含有α、β型糖苷键以及羰基、羧基、醛基、羟基等官能团，具有三螺旋结构，微观结构呈片状。该多糖的热稳定性较强且其溶液为假塑性流体。SBP具有较强的体外抗氧化功效，其中，对DPPH自由基的清除效果较强。此外，沙棘果多糖对游离Fe²⁺也有较好的螯合作用效果。综上，沙棘果多糖具有开发为功能性食品添加剂的巨大潜力。
- 沙棘果 /
- 多糖 /
- 理化特征 /
- 结构特征 /
- 抗氧化
Abstract: In this study, a polysaccharide was extracted and separated from seabuckthorn fruits by water extraction. The physicochemical characteristics were characterized, including monosaccharide composition, molecular weight, microscopic characterization, thermal stability, and rheological properties. Four models were used to evaluate its antioxidant activity in vitro. The results showed that the main component of the polysaccharide was an acidic pectin polysaccharide with a molecular weight of 37.82×10⁴ Da. It was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, xylose, and glucuronic acid with a molar ratio of 0.244: 0.098: 0.265: 0.075: 0.091: 0.081: 0.103. It also contained α- and β-glycoside bonds and carbonyl, carboxyl, aldehyde and hydroxyl groups, with three spiral and lamellar structures. The polysaccharide had strong thermal stability whose solution exhibited pseudoplastic properties. Seabuckthorn berry polysaccharide had a strong antioxidant capacity in vitro and showed significant scavenging ability on DPPH free radicals. In addition, it presented chelating ability on Fe²⁺. In conclusion, seabuckthorn fruit polysaccharide has the potential to serve as functional food additives.
- seabuckthorn berry /
- polysaccharides /
- physicochemical characteristic /
- structure characteristic /
- antioxidant

HTML全文

图 1 SBP的分子量组成

Figure 1. Molecular weight distribution of SBP

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图 2 SBP的单糖组成

Figure 2. Monosaccharide composition of SBP

注：A：单糖标准品；B：SBP。

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图 3 SBP红外光谱图

Figure 3. Infra-red spectrogram of SBP

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图 4 SBP的扫描电镜微观结构

Figure 4. SEM microstructures of SBP

注：A：500×；B：2000×；C：5000×。

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图 5 SBP三螺旋结构的测定

Figure 5. Determination of triple helix structure of SBP

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图 6 SBP的热重特性

Figure 6. Thermogravimetric properties of SBP

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图 7 SBP的流变特性

Figure 7. Rheological properties of SBP

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图 8 SBP的Fe²⁺螯合能力

Figure 8. Fe²⁺ chelating ability of SBP

注：不同小写字母表示差异显著（P<0.05）；图9~图11同。

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图 9 SBP的FRAP还原力

Figure 9. FRAP reducing power of SBP

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图 10 SBP的ABTS⁺自由基清除效果

Figure 10. ABTS⁺ free radical scavenging effect of SBP

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图 11 SBP的DPPH自由基清除效果

Figure 11. DPPH free radical scavenging effect of SBP

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表 1 单糖标准母液的配制

Table 1. Preparation of monosaccharide standard mother solution

单糖标准品	岩藻糖	盐酸氨基半乳糖	鼠李糖	阿拉伯糖	盐酸氨基葡萄糖	半乳糖	葡萄糖	N-乙酰-D-氨基葡萄糖	木糖	甘露糖	果糖	核糖	半乳糖醛酸	古罗糖醛酸	葡萄糖醛酸	甘露糖醛酸
浓度（mmol/L）	0.030	0.014	0.027	0.025	0.023	0.028	0.028	0.022	0.033	0.028	0.083	0.067	0.026	0.052	0.026	0.052

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表 2 流动相比例随时间的变化

Table 2. Change of mobile phases ratio upon time

时间（min）	A（%）	B（%）	C（%）
0	98.8	1.2	0
18.0	98.8	1.2	0
20.0	50	50	0
30.0	50	50	0
30.1	0	0	100
46.0	0	0	100
46.1	0	100	0
50.0	0	100	0
50.1	98.8	1.2	0
60.0	98.8	1.2	0
75.0	98.8	1.2	0

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表 3 SBP的分子量组成

Table 3. Molecular weight composition of SBP

峰序号	出峰时间（min）	Mw（Da）	峰面积比（%）
1	34.338	378199	87.576
2	42.543	10762	8.919
3	43.764	6337	3.505

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表 4 SBP的单糖组成

Table 4. Monosaccharide composition of SBP

单糖名称	简称	摩尔比
岩藻糖	Fuc	0.244
盐酸氨基半乳糖	GalN	0.026
鼠李糖	Rha	0.098
阿拉伯糖	Ara	0.265
盐酸氨基葡萄糖	GlcN	0.008
半乳糖	Gal	0.075
葡萄糖	Glc	0.091
N-乙酰-D-氨基葡萄糖	GlcNac	0.008
木糖	Xyl	0.081
葡萄糖醛酸	GlcA	0.103

下载: 导出CSV

参考文献(50)

[1]	崔立柱, 付依依, 刘士伟, 等. 沙棘营养价值及产业发展概况[J]. 食品研究与开发,2021,42(11):218−224. [CUI L Z, FU Y Y, LIU S W, et al. Nutritional value and industry development of sea buckthorn[J]. Food Research and Development,2021,42(11):218−224. doi: 10.12161/j.issn.1005-6521.2021.11.034 CUI L Z, FU Y Y, LIU S W, et al. Nutritional value and industry development of sea buckthorn[J]. Food Research and Development, 2021, 42(11): 218-224. doi: 10.12161/j.issn.1005-6521.2021.11.034
[2]	刘勇, 廉永善, 王颖莉, 等. 沙棘的研究开发评述及其重要意义[J]. 中国中药杂志,2014,39(9):1547−1552. [LIU Y, LIAN Y S, WANG Y L, et al. Review of research and development and significant effect of Hippophae rhamnoides[J]. China Journal of Chinese,2014,39(9):1547−1552. LIU Y, LIAN Y S, WANG Y L, et al. Review of research and development and significant effect of Hippophae rhamnoides[J]. China Journal of Chinese , 2014, 39(9): 1547-1552.
[3]	王萌, 王子纯, 黄京美, 等. 沙棘黄酮类物质提取纯化及功能活性研究进展[J]. 新宝登录入口(中国)有限公司,2023,44(2):487−496. [WANG M, WANG Z C, HUANG J M, et al. Research progress on extraction, purification and functional activity of seabuckthorn flavonoids[J]. Science and Technology of Food Industry,2023,44(2):487−496. WANG M, WANG Z C, HUANG J M, et al. Research progress on extraction, purification and functional activity of seabuckthorn flavonoids[J/OL]. Science and Technology of Food Industry, 2023, 44(02): 487-496.
[4]	NIYIGABA T, LIU D, HABIMANA J D. The extraction, functionalities and applications of plant polysaccharides in fermented foods: A review[J]. Foods, 2021, 10(12): 3004-3004.
[5]	LIU J F, XU D X, CHEN S, et al. Superfruits in China: Bioactive phytochemicals and their potential health benefits-A review[J]. Food Sci Nutr,2021,9(12):6892−6902. doi: 10.1002/fsn3.2614
[6]	REN R R, LI N, SU C, et al. The bioactive components as well as the nutritional and health effects of sea buckthorn[J]. Rsc Adv,2020,10(73):44654−44671. doi: 10.1039/D0RA06488B
[7]	ZHAO L, LI M Y, SU S, et al. Hippophae rhamnoides L. polysaccharide enhances antioxidant enzyme activity, cytokine level, and related mRNA expression in intestinal porcine epithelial cells[J]. Can J Anim Sci,2020,100(1):193−204. doi: 10.1139/cjas-2019-0134
[8]	ZHAO S J, SUN H Y, LIU Q B, et al. Protective effect of seabuckthorn berry juice against acrylamide-induced oxidative damage in rats[J]. J Food Sci,2020,85(7):2245−2254. doi: 10.1111/1750-3841.15313
[9]	NI W H, GAO T T, WANG H L, et al. Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants[J]. J Ethnopharmacol,2013,150(2):529−535. doi: 10.1016/j.jep.2013.08.055
[10]	王瑞琴, 杨晓光, 许文婷, 等. 沙棘多糖的研究进展[J]. 食品研究与开发,2017,38(13):221−224. [WANG R Q, YANG X G, XU W T, et al. Research progress of sea-buckthorn polysaccharide[J]. Food Research and Development,2017,38(13):221−224. doi: 10.3969/j.issn.1005-6521.2017.13.048 WANG R Q, YANG X G, XU W T, et al. Research progress of sea-buckthorn polysaccharide[J]. Food Research and Development, 2017, 38(13): 221-224. doi: 10.3969/j.issn.1005-6521.2017.13.048
[11]	ZHANG W, ZHANG X, ZOU K, et al. Seabuckthorn berry polysaccharide protects against carbon tetrachloride-induced hepatotoxicity in mice via anti-oxidative and anti-inflammatory activities[J]. Food & Function,2017,8(9):3130−3138.
[12]	杜晶晶, 杨宏志. 沙棘多糖抗氧化活性的研究[J]. 黑龙江八一农垦大学学报,2011,23(2):49−52. [DU J J, YANG H Z. Study on antioxidation activities of sea-buckthorn crude polysaccharide[J]. Journal of Heilongjiang BayiAgricultural University,2011,23(2):49−52. doi: 10.3969/j.issn.1002-2090.2011.02.014 DU J J, YANG H Z. Study on antioxidation activities of sea-buckthorn crude polysaccharide[J]. Journal of Heilongjiang BayiAgricultural University, 2011, 23(2): 49-52. doi: 10.3969/j.issn.1002-2090.2011.02.014
[13]	WANG Z J, XIE J H, NIE S P, et al. Review on cell models to evaluate the potential antioxidant activity of polysaccharides[J]. Food & Function,2017,8(3):915−926.
[14]	ZHONG Q, WEI B, WANG S, et al. The antioxidant activity of polysaccharides derived from marine organisms: An overview[J]. Mar Drugs,2019,17(12):674. doi: 10.3390/md17120674
[15]	钟运翠. 沙棘果渣中水溶性多糖的提取、分离、纯化及结构初探 [D]. 大庆: 黑龙江八一农垦大学, 2009. ZHONG Y C. Extraction, separation, purification and structure preliminary exploration of water-soluble polysaccharides from pomace of sea-buckthorn[D]. Daqing: Heilongjiang Bayi Agricultural University, 2009.
[16]	杨宏志, 王洪江, 张春芝, 等. 热水提取沙棘多糖工艺研究[J]. 农产品加工,2018(3):22−25. [YANG H Z, WANG H J, ZHANG C Z, et al. Study on extraction technology of polysaccharides from sea-buckthorn by hot-water processing[J]. Farm Products Processing,2018(3):22−25. YANG H Z, WANG H J, ZHANG C Z, et al. Study on extraction technology of polysaccharides from sea-buckthorn by hot-water processing [J]. Farm Products Processing, 2018 (3): 22-25.
[17]	关奇, 杨万政, 温中平. 沙棘果皮、叶中多糖的提取及其抑菌作用研究[J]. 国际沙棘研究与开发,2005(2):17−20. [GUAN Q, YANG W Z, WEN Z P. Extracti on of polysaccharide fro m seabuckthorn pericarp and leaves and its bacteriostasis research[J]. Water Resources Development and Management,2005(2):17−20. GUAN Q, YANG W Z, WEN Z P. Extracti on of polysaccharide fro m seabuckthorn pericarp and leaves and its bacteriostasis research[J]. Water Resources Development and Management, 2005(2): 17-20
[18]	杨林莎, 李玉贤, 李明丽, 等. 苯酚-硫酸比色法测定百合多糖的含量[J]. 中国中医药信息杂志,2004(8):704−705. [YANG S L, LI Y X, LI M L, et al. Determination for the content of polysaccharides in the Lilium brownii by phenol-vitriolic[J]. Chinese Journal of Information on Traditional Chinese Medicine,2004(8):704−705. doi: 10.3969/j.issn.1005-5304.2004.08.022 YANG S L, LI Y X, LI M L, et al. Determination for the content of polysaccharides in the Lilium brownii by phenol-vitriolic[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2004(8): 704-705. doi: 10.3969/j.issn.1005-5304.2004.08.022
[19]	徐亚, 范会芬, 赵玎玲, 等. 考马斯亮蓝法测定大豆水溶性蛋白提取方法的优化[J]. 大豆科学,2022,41(2):196−202. [XU Y, FAN H F, ZHAO D L, et al. Optimization of extraction method for water-soluble protein determination by coomassie bright blue method[J]. Soybean Science,2022,41(2):196−202. doi: 10.11861/j.issn.1000-9841.2022.02.0196 XU Y, FAN H F, ZHAO D L, et al. Optimization of extraction method for water-soluble protein determination by coomassie bright blue method[J]. Soybean Science, 2022, 41(2): 196-202. doi: 10.11861/j.issn.1000-9841.2022.02.0196
[20]	方青青. 油茶桑寄生多糖的提取纯化、结构分析及其生物活性研究 [D]. 湘潭: 湘潭大学, 2019. FANG Q Q. The Extraction, purification, structural analysis and bioactivity research of polysaccharide from mistletoe of Camellia oleifera[D]. Xiangtan: Xiangtan University, 2019.
[21]	何纤, 李俊年, 杨冬梅, 等. 马蹄皮多酚的抗氧化活性及其对HepG-2细胞增殖的抑制[J]. 华西药学杂志,2021,36(6):628−632. [HE X, LI J N, YANG D M, et al. Antioxidant activity and inhibitive effect on HepG-2 cells proliferation of polyphenol from water chestnut peel[J]. West China Journal of Pharmaceutical Sciences,2021,36(6):628−632. doi: 10.13375/j.cnki.wcjps.2021.06.006 HE X, LI J N, YANG D M, et al. Antioxidant activity and inhibitive effect on HepG-2 cells proliferation of polyphenol from water chestnut peel[J]. West China Journal of Pharmaceutical Sciences, 2021, 36(6): 628-632. doi: 10.13375/j.cnki.wcjps.2021.06.006
[22]	GUAN X, WANG Q, LIN B, et al. Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages[J]. International Journal of Biological Macromolecules,2022,217:131−41. doi: 10.1016/j.ijbiomac.2022.07.043
[23]	HUANG S T, TENG N C, WANG H H, et al. Wasted ganoderma tsugae derived chitosans for smear layer removal in endodontic treatment[J]. Polymers (Basel),2019,11(11):1795. doi: 10.3390/polym11111795
[24]	王苗苗, 刘宗浩, 张永, 等. 2种新疆沙棘中黄酮、多酚及其抗氧化活性分析[J]. 新宝登录入口(中国)有限公司,2020,41(18):51−57. [WANG M M, LIU Z H, ZHANG R, et al. Analysis of the flavonoid, polyphenol and its antioxidant activity of 2 kinds of sea buckthorn from Xinjiang[J]. Science and Technology of Food Industry,2020,41(18):51−57. doi: 10.13386/j.issn1002-0306.2020.18.008 WANG M M, LIU Z H, ZHANG R, et al. Analysis of the flavonoid, polyphenol and its antioxidant activity of 2 kinds of sea buckthorn from xinjiang[J]. Science and Technology of Food Industry, 2020, 41(18): 51-57. doi: 10.13386/j.issn1002-0306.2020.18.008
[25]	韦志, 阮心眉, 戴涛涛, 等. 碱提砂仁多糖的结构表征及其抗氧化活性研究[J]. 新宝登录入口(中国)有限公司,2021,42(24):87−93. [WEI Z, RUAN X M, DAI T T, et al. Structure characterization and antioxidant activity of polysaccharides from Amomum villosum extracted with alkaline solution[J]. Science and Technology of Food Industry,2021,42(24):87−93. WEI Z, RUAN X M, DAI T T, et al. Structure characterization and antioxidant activity of polysaccharides from Amomum villosum extracted with alkaline solution[J]. Science and Technology of Food Industry, 2021, 42(24): 87-93.
[26]	金婷. 沙棘多糖的提取纯化、结构鉴定及其抗氧化性的研究 [D]. 哈尔滨: 东北农业大学, 2006: 43. JIN T. Studies on extruction, purification, structure analysis and antioxidative effect of the polysaccharides in Hippophae rhamoides L.[D]. Harbin: Northeast Agricultural University, 2006: 43.
[27]	蔡菲, 安美忱, 刘安妮. 超声波法优化沙棘果实多糖及结构的初步研究[J]. 农产品加工,2018(5):13−16, 20. [CAI F, AN M C, LIU A N. Studies on isolation by ultrasonic-assisted and structure of polysaccharides from Hippophae rhamoides L doi: 10.16693/j.cnki.1671-9646(X).2018.03.005 J]. Farm Products Processing,2018(5):13−16, 20. doi: 10.16693/j.cnki.1671-9646(X).2018.03.005
[28]	王纯玮, 白英. 开菲尔胞外多糖理化性质及抗氧化特性研究[J]. 食品与发酵工业,2022,48(21):104−110. [WANG C W, BAI Y. Study on physicochemical properties and antioxidant properties of Kefiran[J]. Food and Fermentation Industries,2022,48(21):104−110. WANG C W, BAI Y. Study on physicochemical properties and antioxidant properties of Kefiran[J]. Food and Fermentation Industries , 2022, 48(21): 104-110.
[29]	WANG J Z, WANG T. How to interpret infrared (IR) spectra[J]. University Chemistry,2016,31(6):90−97. doi: 10.3866/PKU.DXHX201504001
[30]	崔倩倩, 孙志伟, 张长城, 等. 竹节参多糖的免疫佐剂活性研究[J]. 中国中医药信息杂志,2014,21(12):88−91. [CUI Q Q, SUN Z W, ZHANG C C, et al. Study on adjuvant immunoactivity of polysaccharides from Panax japonicus[J]. Chinese Journal of Information on Traditional Chinese Medicine,2014,21(12):88−91. doi: 10.3969/j.issn.1005-5304.2014.12.026 CUI Q Q, SUN Z W, ZHANG C C, et al. Study on adjuvant immunoactivity of polysaccharides from Panax japonicus[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2014, 21(12): 88-91. doi: 10.3969/j.issn.1005-5304.2014.12.026
[31]	刘阿娟, 张静, 张化朋, 等. 虎奶菇菌核多糖SHNP的分离纯化及形貌观测[J]. 农产品加工(学刊),2013(18):66−69. [LIU A J, ZHANG J, ZHANG H P, et al. Purification and atomic force microscope observationof SHNP from the mushroom sclerotia of Pleurotus tuber-regium[J]. Academic Periodical of Farm Products Processing,2013(18):66−69. LIU A J, ZHANG J, ZHANG H P, et al. Purification and atomic force microscope observationof SHNP from the mushroom sclerotia of Pleurotus tuber-regium[J]. Academic Periodical of Farm Products Processing, 2013(18): 66-69.
[32]	魏晨业. 沙棘多糖分离纯化及生物活性研究[D]. 乌鲁木齐: 新疆农业大学, 2021. WEI C Y. Isolation, purification and bioactivity of seabuckthorn polysaccharides[D]. Urumqi: Xinjiang Agricultural University, 2021.
[33]	刘宏, 陈晓琳, 孙雨豪, 等. 蜈蚣藻多糖的降解及其体外抗氧化活性研究[J]. 海洋科学,2020,44(8):186−196. [LIU H, CHEN X H, SUN Y H, et al. Degradation of sulfated polysaccharides from Grateloupia filicina and their antioxidant activities in vitro[J]. Marine Sciences,2020,44(8):186−196. LIU H, CHEN X H, SUN Y H, et al. Degradation of sulfated polysaccharides from Grateloupia filicina and their antioxidant activities in vitro[J]. Marine Sciences, 2020, 44(8): 186-196.
[34]	刘青业, 许小娟. 三螺旋多糖的链结构与功能研究进展[J]. 功能高分子学报,2016,29(2):134−152. [LIU Q Y, XU X J. Recent progress in China conformation and function triple helical polysaccharides[J]. Journal of Functional Polymers,2016,29(2):134−152. doi: 10.14133/j.cnki.1008-9357.2016.02.002 LIU Q Y, XU X J. Recent progress in China conformation and function triple helical polysaccharides[J]. Journal of Functional Polymers, 2016, 29(2): 134-152. doi: 10.14133/j.cnki.1008-9357.2016.02.002
[35]	吴少微. 鸡骨草多糖的生物活性、结构解析及快速制备活性多糖的研究[D]. 广州: 华南理工大学, 2016: 67. WU S W. Biological activities and structural characteristics of polysaccharides from Abrus cantoniensis and the rapid preparation for the bioactive polysaccharide[D]. Guangzhou: South China University of Technology, 2016: 67.
[36]	滕浩, 曾媚, 王敬涵, 等. 西番莲果皮多糖的提取、结构鉴定及流变性分析[J]. 食品科技,2021,46(10):189−193. [TENG H, ZENG M, WANG J H, et al. Extraction, structural identification and rheological analysis of polysaccharide from Passiflora edulis Sims peel[J]. Food Science and Technology,2021,46(10):189−193. doi: 10.3969/j.issn.1005-9989.2021.10.spkj202110030 TENG H, ZENG M, WANG J H, et al. Extraction, structural identification and rheological analysis of polysaccharide from Passiflora edulis Sims peel[J]. Food Science and Technology, 2021, 46(10): 189-193. doi: 10.3969/j.issn.1005-9989.2021.10.spkj202110030
[37]	吴潇霞, 陈楠, 白冰瑶, 等. 大果白刺多糖的物理特性及抗氧化活性研究[J]. 新宝登录入口(中国)有限公司,2021,42(23):87−94. [WU X X, CHEN N, BAI B Y, et al. Physical properties and antioxidant activity of polysaccharide from Nitraria roborowskii Kom fruit[J]. Science and Technology of Food Industry,2021,42(23):87−94. doi: 10.13386/j.issn1002-0306.2021040312 WU X X, CHEN N, BAI B Y, et al. Physical properties and antioxidant activity of polysaccharide from Nitraria roborowskii Kom fruit[J]. Science and Technology of Food Industry, 2021, 42(23): 87-94. doi: 10.13386/j.issn1002-0306.2021040312
[38]	吕喜茹, 郭亮, 常明昌, 等. 姬松茸粗多糖抗氧化作用[J]. 食用菌学报,2010,17(1):69−71. [LÜ X R, GUO L, CHANG M C, et al. antioxidant activity of polysaccharide from Agaricus blazei Murill[J]. Acta Edulis Fungi,2010,17(1):69−71. doi: 10.3969/j.issn.1005-9873.2010.01.013 LV X R, GUO L, CHANG M C, et al. antioxidant activity of polysaccharide from Agaricus blazei Murill[J]. Acta Edulis Fungi, 2010, 17(1): 69-71. doi: 10.3969/j.issn.1005-9873.2010.01.013
[39]	任薇, 包晓玮, 张志芳, 等. 沙棘多糖清除自由基及抗脂质过氧化作用研究[J]. 新宝登录入口(中国)有限公司,2019,40(8):272−277. [REN W, BAO X W, ZHANG Z F, elt. Study on free radical scavenging and anti-lipid peroxidation of seabuckthorn polysaccharide[J]. Science and Technology of Food Industry,2019,40(8):272−277. doi: 10.13386/j.issn1002-0306.2019.08.046 REN W, BAO X W, ZHANG Z F, HAN L, elt. Study on free radical scavenging and anti-lipid peroxidation of seabuckthorn polysaccharide[J]. Science and Technology of Food Industry, 2019, 40(8): 272-277. doi: 10.13386/j.issn1002-0306.2019.08.046
[40]	魏晨业, 包晓玮, 王娟, 等. 沙棘多糖分离纯化及抗氧化活性[J]. 食品科学,2021,42(4):227−232. [WEI C Y, BAO X W, WANG J, et al. Isolation, purification and antioxidant activity of polysaccharides from the fruit of Hippophae rhamnoides[J]. Food Science,2021,42(4):227−232. WEI C Y, BAO X W, WANG J, et al Isolation, purification and antioxidant activity of polysaccharides from the fruit of Hippophae rhamnoides[J]. Food Science, 2021, 42(4): 227-232.
[41]	WANG H, LIU Y M, QI Z M, et al. An overview on natural polysaccharides with antioxidant properties[J]. Current Medicinal Chemistry,2013,20(23):2899−2913. doi: 10.2174/0929867311320230006
[42]	WANG J, HU S, NIE S, et al. Reviews on mechanisms of in vitro antioxidant activity of polysaccharides[J]. Oxid Med Cell Longev,2016,2016:5692852.
[43]	WEI E, YANG R, ZHAO H, et al. Microwave-assisted extraction releases the antioxidant polysaccharides from seabuckthorn (Hippophae rhamnoides L.) berries[J]. Int J Biol Macromol,2019,123:280−290. doi: 10.1016/j.ijbiomac.2018.11.074
[44]	王帅, 赵冬雪, 韩成凤, 等. 6种活性多糖的结构、性质及其抗氧化活性的比较研究[J]. 食品研究与开发,2021,42(16):7−15. [WANG S, ZHAO D X, HAN C F, et al. A comparative study on the structure, properties and antioxidant activity of six active polysaccharides[J]. Food Research and Development,2021,42(16):7−15. doi: 10.12161/j.issn.1005-6521.2021.16.002 WANG S, ZHAO D X, HAN C F, et al. A comparative study on the structure, properties and antioxidant activity of six active polysaccharides[J]. Food Research and Development, 2021, 42(16): 7-15. doi: 10.12161/j.issn.1005-6521.2021.16.002
[45]	王通, 王广飞, 张淑敏, 等. 基于天然多糖的水凝胶伤口敷料的研究进展[J]. 材料导报,2022,36(6):182−190. [WANG T, WANG G F, ZHANG S M, et al. Research progress of hydrogel wound dressing based on natural polysaccharides[J]. Materials Reports,2022,36(6):182−190. doi: 10.11896/cldb.20060050 WANG T, WANG G F, ZHANG S M, et al. Research progress of hydrogel wound dressing based on natural polysaccharides[J]. Materials Reports, 2022, 36(6): 182-190. doi: 10.11896/cldb.20060050
[46]	韦阳, 王迪, 孙翠霞, 等. 基于生物大分子的纳米颗粒传递体系研究进展[J]. 中国食品添加剂,2017(10):167−176. [WEI Y, WANG D, SUN C X, et al. Research progress of biomacromolecular nanoparticle delivery systems[J]. China Food Additives,2017(10):167−176. doi: 10.3969/j.issn.1006-2513.2017.10.023 WEI Y, WANG D, SUN C X, et al. Research progress of biomacromolecular nanoparticle delivery systems[J]. China Food Additives, 2017(10): 167-176. doi: 10.3969/j.issn.1006-2513.2017.10.023
[47]	马航宇, 张士凯, 张可欣, 等. 果胶微胶囊技术研究及应用进展[J]. 饮料工业,2021,24(4):71−78. [MA H Y, ZHANG S K, ZHANG K X, et al. Microencapsulation technology research and application progress of pectin[J]. Beverage Industry,2021,24(4):71−78. doi: 10.3969/j.issn.1007-7871.2021.04.016 MA H Y, ZHANG S K, ZHANG K X, et al. Microencapsulation technology research and application progress of pectin[J]. Beverage Industry, 2021, 24(4): 71-78. doi: 10.3969/j.issn.1007-7871.2021.04.016
[48]	黄金, 崔朝经, 韩雪, 等. 多糖纳米材料制备、表征及在果蔬可食性涂膜中应用的研究进展[J]. 新宝登录入口(中国)有限公司,2021,42(24):424−433. [HUANG J, CUI C J, HAN X, et al. Research progress on preparation, characterization and application of polysaccharide nanomaterials in edible coating of fruits and vegetables[J]. Science and Technology of Food Industry,2021,42(24):424−433. doi: 10.13386/j.issn1002-0306.2020110068 HUANG J, CUI C J, HAN X, et al. Research progress on preparation, characterization and application of polysaccharide nanomaterials in edible coating of fruits and vegetables[J]. Science and Technology of Food Industry, 2021, 42(24): 424-433. doi: 10.13386/j.issn1002-0306.2020110068
[49]	PANAHIRAD S, DADPOUR M, PEIGHAMBARDOUST S H, et al. Applications of carboxymethyl cellulose- and pectin-based active edible coatings in preservation of fruits and vegetables: A review[J]. Trends in Food Science & Technology,2021,110:663−673.
[50]	LEE H B, KIM Y S, PARK H Y. Pectic polysaccharides: Targeting gut microbiota in obesity and intestinal health[J]. Carbohydr Polym,2022,287:119363. doi: 10.1016/j.carbpol.2022.119363