响应面法优化西洋参果多糖的提取工艺及其体外抗氧化活性

赵丽明; 郭煦遥; 毛英民; 赵大庆; 黄宝泰; 李佳奇; 刘莉; 齐滨

doi:10.13386/j.issn1002-0306.2022070318

响应面法优化西洋参果多糖的提取工艺及其体外抗氧化活性

doi: 10.13386/j.issn1002-0306.2022070318

赵丽明^1,,
郭煦遥²,
毛英民¹,
赵大庆¹,
黄宝泰²,
李佳奇¹,
刘莉^2, ,,
齐滨^2, ,

1.
长春中医药大学吉林省人参科学研究院，吉林长春 130117
2.
长春中医药大学药学院，吉林长春 130117

基金项目: 国家重点研发计划（2021YFD1600903）；吉林省科技厅重点研发项目（20210204062YY）；吉林省大学生创新创业训练计划项目（S202110199138X）。

详细信息

作者简介:
赵丽明（1998−），女，硕士研究生，研究方向：中药有效成分发现、生物转化及健康产品研究与开发，E-mail：2139994228@qq.com

通讯作者:
刘莉（1978−），女，博士，副教授，研究方向：中药活性物质分析研究，E-mail：68734263@qq.com

齐滨（1978−），男，博士，教授，研究方向：天然产物开发与研究，E-mail：qibin88@126.com

中图分类号: TS201.2
计量
- 文章访问数: 45
- HTML全文浏览量: 13
- PDF下载量: 9
- 被引次数: 0
出版历程
- 收稿日期: 2022-08-02
- 网络出版日期: 2023-05-21
- 刊出日期: 2023-07-01

Optimization of Extraction Process and Antioxidant Activity of Polysaccharide from Panax quinquefolium Fruit by Response Surface Methodology

Liming ZHAO^1
,,
Xuyao GUO²,
Yingmin MAO¹,
Daqing ZHAO¹,
Baotai HUANG²,
Jiaqi LI¹,
Li LIU^{2
, ,},
Bin QI^{2
, ,}

1.
Institute of Ginseng Science, Changchun University of TCM, Changchun 130117, China
2.
School of Pharmacy, Changchun University of TCM, Changchun 130117, China

摘要

摘要: 目的：对西洋参果实中的多糖进行提取，结合响应面法对提取工艺进行优化，并对西洋参果多糖是否具有体外抗氧化活性进行研究。方法：本研究以新鲜的西洋参果实为原料，采用了水提醇沉法提取其中的多糖。用单因素实验以及响应面法对提取工艺进行了优化。从DPPH自由基清除率、羟基自由基清除率以及还原能力三个方面进行果多糖的体外抗氧化活性研究。结果：最佳工艺参数为：提取时间为2.5 h，乙醇浓度为80%，料液比为1:16 g/mL，此时的多糖得率为29.47%±0.65%，与模型预测值相当。在以下三方面考察了西洋参果多糖的体外抗氧化活性：多糖浓度为3.4 mg/mL时，其DPPH自由基清除率达75.14%±0.65%，IC₅₀值为0.71 mg/mL；多糖浓度为3.4 mg/mL时，其羟基自由基的清除率可达71.82%±1.43%，IC₅₀值为0.87 mg/mL；多糖的浓度为1.0 mg/mL时，其总还原力达到了0.730，并且其体外抗氧化能力随西洋参果多糖浓度的增加而增强。结论：抗氧化活性的实验结果说明了西洋参果多糖具有较好的抗氧化活性。本研究可以为西洋参果多糖进一步开发利用奠定理论基础，确保中药资源能够被充分利用。
- 西洋参 /
- 多糖 /
- 提取工艺 /
- 响应面优化 /
- 抗氧化活性
Abstract: Objective: For the extraction of polysaccharides from American ginseng fruits, the extraction process was optimized in combination with response surface methodology, and to investigate whether polysaccharides from American ginseng fruits had antioxidant activity in vitro. Methods: In this study, polysaccharides were extracted from fresh American ginseng fruits by water extraction and alcohol precipitation method. The extraction process was optimized using single factor tests as well as response surface methodology. In vitro antioxidant activity studies of polysaccharides from American ginseng fruits were conducted from three aspects: DPPH radical scavenging rate, hydroxyl radical scavenging rate as well as reducing power. Results: The optimal process parameters were as follows: the extraction time was 2.5 h, the concentration of ethanol was 80%, the feed to liquid ratio of 1:16 g/mL, and the measured average value of polysaccharide yield was 29.47%±0.65%, which was comparable to the model predicted value. In vitro antioxidant activities of polysaccharides from American ginseng fruits were examined in the following three aspects. When the concentration of polysaccharides was 3.4 mg/mL, the DPPH radical scavenging rate was 75.14%±0.65%, and IC₅₀ value was 0.71 mg/mL. When the concentration of polysaccharide was 3.4 mg/mL, the scavenging rate against hydroxyl radicals could reach 71.82%±1.43%, and IC₅₀ value was 0.87 mg/mL. When the concentration of polysaccharides was 1.0 mg/mL, the total reducing power reached 0.730, and its in vitro antioxidant capacity was enhanced with increasing concentrations of polysaccharides from American ginseng fruits. Conclusion: The experimental results of antioxidant activities illustrated that polysaccharides from American ginseng fruits had better antioxidant activities. This study may provide a theoretical basis for further development and utilization of polysaccharides from American ginseng fruits, and ensure that traditional Chinese medicine resources can be fully utilized.
- Panax quinquefolium L /
- polysaccharide /
- extraction process /
- response surface methodology /
- antioxidant activity

HTML全文

图 1 提取时间对西洋参果多糖得率的影响

Figure 1. Effect of extraction time on the yield of polysaccharides from American ginseng fruits

下载: 全尺寸图片幻灯片

图 2 乙醇浓度对西洋参果多糖得率的影响

Figure 2. Effect of ethanol concentration on the yield of polysaccharides from American ginseng fruits

下载: 全尺寸图片幻灯片

图 3 料液比对西洋参果多糖得率的影响

Figure 3. Effect of ratio of solid to liquid on polysaccharide yield of American ginseng fruits

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图 4 AB、AC、BC对西洋参果多糖得率影响的响应面图和等高线图

Figure 4. Response surface plots and contour plots of the effects of AB, AC and BC on polysaccharide yield of American ginseng fruit

下载: 全尺寸图片幻灯片

图 5 西洋参果多糖对DPPH自由基清除率

Figure 5. Scavenging rate of American ginseng fruit polysaccharide on DPPH radical

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图 6 西洋参果多糖对·OH清除率

Figure 6. Scavenging rate of American ginseng fruit polysaccharide on hydroxyl radical

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图 7 西洋参果多糖总还原力

Figure 7. Total reduction force of American ginseng fruit polysaccharide

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表 1 试验因素水平表

Table 1. Table of experimental factor levels

水平	因素
水平	A提取时间（h）	B乙醇浓度（%）	C料液比（g/mL）
−1	1	75	1:10
0	2	80	1:15
1	3	85	1:20

下载: 导出CSV

表 2 Box-Behnken试验设计方案及结果

Table 2. Design and test results of Box-Behnken test

试验号	提取时间	乙醇浓度	料液比	西洋参果多糖得率（%）
1	1	1	0	25.43±0.63
2	0	0	0	27.54±0.93
3	0	1	1	26.72±0.80
4	1	0	−1	26.65±0.57
5	1	0	1	26.28±0.40
6	0	−1	1	24.83±1.72
7	0	0	0	27.75±0.63
8	0	−1	−1	22.03±1.17
9	−1	0	−1	14.64±1.91
10	0	1	−1	23.45±0.86
11	1	−1	0	24.83±0.90
12	0	0	0	28.75±0.59
13	−1	0	1	15.24±0.84
14	0	0	0	28.62±0.75
15	0	0	0	27.58±1.07
16	−1	1	0	15.15±0.40
17	−1	−1	0	14.35±0.63

下载: 导出CSV

表 3 回归模型方差分析

Table 3. Variance analysis of response surface quadratic regression

方差来源	平方和	自由度	均方	F值	P值
模型	437.07	9	48.56	51.00	<0.0001^**
A-提取时间	240.90	1	240.90	252.99	<0.0001^**
B-乙醇浓度	2.76	1	2.76	2.90	0.1324
C-料液比	5.12	1	5.12	5.38	0.0535
AB	1.21	1	1.21	0.011	0.9213
AC	0.20	1	0.20	0.21	0.6587
BC	0.063	1	0.063	0.066	0.8052
A²	143.36	1	143.36	150.55	<0.0001^**
B²	21.98	1	21.98	23.09	0.0020^**
C²	9.60	1	9.60	10.08	0.0156^*
残差	6.67	7	0.95
失拟项	5.32	3	1.77	5.26	0.0713
纯误差	1.35	4	0.34
总离差	443.74	16
注：^*表示差异极显著P<0.01，^表示差异显著P<0.05。

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