Analysis of Functional Components of Kangding Ganoderma leucocontextum and Its Antioxidant and Hypoglycemic Activity in Virto
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摘要: 本文采用药典方法和高效液相色谱法分析了康定灵芝多糖、三萜及灵芝酸A、灵芝酸C1、灵芝酸F的含量。通过对DPPH、ABTS+自由基清除能力和总抗氧化能力的考察以及对α-淀粉酶和α-葡萄糖苷酶活性的抑制效果评价了其体外抗氧化和降血糖活性。结果显示，康定灵芝多糖和三萜含量分别为1.15%和1.50%，灵芝酸A、灵芝酸C1和灵芝酸F含量分别为0.052%、0.020%和0.064%。体外抗氧化和降血糖活性评价结果表明康定灵芝多糖提取物对DPPH和ABTS+自由基清除的IC50值分别为0.75 mg/mL和1.24 mg/mL，对α-淀粉酶和α-葡萄糖苷酶抑制的IC50值分别为1.45 mg/mL和1.97 mg/mL。三萜提取物对DPPH和ABTS+自由基清除的IC50值分别为0.65 mg/mL和1.06 mg/mL，对α-淀粉酶和α-葡萄糖苷酶抑制的IC50值分别为1.44 mg/mL和1.09 mg/mL。本文研究结果表明康定灵芝功效成分含量高，同时具有良好的抗氧化和降血糖活性，这将为康定灵芝在食品、药品和保健品等方面的开发利用提供理论基础和参考依据。Abstract: The contents of polysaccharides, triterpenes, ganoderic acid A, ganoderic acid C1 and ganoderic acid F in Kangding Ganoderma leucocontextum were analyzed by pharmacopoeia and HPLC. The antioxidant activities were evaluated by DPPH· and ABTS+· scavenging abilities and total antioxidant capacity, and hypoglycemic activities were evaluated by the inhibitory effect on α-amylase and α-glucosidase activities. The results showed that the contents of polysaccharides and triterpenes in Kangding Ganoderma leucocontextum were 1.15% and 1.50%, and the contents of ganoderic acid A, ganoderic acid C1 and ganoderic acid F were 0.052%, 0.020% and 0.064%, respectively. The antioxidant activities and hypoglycemic activities in vitro showed that IC50 values of polysaccharide extract for DPPH· and ABTS+· scavenging were 0.75 mg/mL and 1.24 mg/mL, and IC50 values of α-amylase and α-glucosidase activities were 1.45 mg/mL and 1.97 mg/mL, respectively. The IC50 values of triterpenoids extract for DPPH· and ABTS+· radical scavenging were 0.65 mg/mL and 1.06 mg/mL, and the IC50 values of α-amylase and α-glucosidase activities were 1.44 mg/mL and 1.09 mg/mL, respectively. The results of this study show that Kangding Ganoderma leucocontextum contain high content of functional components, and presented favourable antioxidant and hypoglycemic activities, which would provide the theoretical basis for the development and utilization of Kangding Ganoderma leucocontextum in food, medicine and health products.
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图 4 康定灵芝多糖及三萜ABTS+自由基清除能力
Figure 4. ABTS+ free radical scavenging ability of Kangding Ganoderma leucocontextum polysaccharide and triterpenoid
图 5 康定灵芝多糖及三萜总抗氧化能力
Figure 5. Total antioxidant capacity of polysaccharides and triterpenoids from Kangding Ganoderma leucocontextum
图 6 康定灵芝多糖及三萜α-淀粉酶活性抑制能力
Figure 6. Inhibitory of α-amylase activity of polysaccharides and triterpene from Kangding Ganoderma leucocontextum
图 7 康定灵芝多糖及三萜α-葡萄糖苷酶活性抑制能力
Figure 7. Inhibitory of α-glucosidase activity of polysaccharide and triterpene from Kangding Ganoderma leucocontextum
表 1 康定灵芝多糖及三萜标准曲线
Table 1. Standard curve of polysaccharides and triterpenoids from Kangding Ganoderma leucocontextum
功效成分 标准曲线 线性范围（μg/mL） R2 多糖 y=0.0058x−0.0026 12~72 0.9991 总三萜 y=0.0263x−0.0602 4~20 0.9988 灵芝酸A y=5.3462x+0.2203 10.42~83.33 0.9994 灵芝酸C1 y=9.2706x+4.1787 5.23~83.33 0.9995 灵芝酸F y=4.3738x−6.5697 10.42~83.33 0.9999 
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表 2 康定灵芝多糖及三萜化合物含量
Table 2. Contents of polysaccharides and triterpenoids in Kangding Ganoderma leucocontextum
成分 含量（%） 多糖 1.150±0.010 总三萜 1.500±0.020 灵芝酸A 0.052±0.001 灵芝酸C1 0.020±0.004 灵芝酸F 0.064±0.006 水分 1.180±0.030
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