装载<i>β</i>-乳球蛋白纳米颗粒的海藻酸钠三层复合膜构建及特性

樊文婷; 李康静; 宋婕; 姜紫菡; 许浩田; 朱俊向; 吴昊

doi:10.13386/j.issn1002-0306.2022070353

装载β-乳球蛋白纳米颗粒的海藻酸钠三层复合膜构建及特性

doi: 10.13386/j.issn1002-0306.2022070353

樊文婷^1,,
李康静¹,
宋婕¹,
姜紫菡¹,
许浩田¹,
朱俊向^{1, 2},
吴昊^{1, 2, ,}

1.
青岛农业大学食品科学与工程学院，山东青岛 266109
2.
青岛特种食品研究院，山东青岛 266109

基金项目: 山东省高等学校国家级大学生创新创业训练计划项目（202110435082）；山东省自然科学基金（ZR2020MC213，ZR2020QC242）；青岛农业大学高层次人才科研基金（6651120039）。

详细信息

作者简介:
樊文婷（2001−），女，本科，研究方向：农产品包装保鲜，E-mail：fan0419wenting@163.com

通讯作者:
吴昊（1981−），女，博士，教授，研究方向：农产品加工及贮藏，E-mail：wuhaoqau@163.com

中图分类号: TS255.1
计量
- 文章访问数: 20
- HTML全文浏览量: 10
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2022-08-02
- 网络出版日期: 2023-05-20
- 刊出日期: 2023-07-01

Construction and Properties of Alginate-based Trilayer Composite Film Loaded with β-Lactoglobulin Nanoparticles

Wenting FAN^1
,,
Kangjing LI¹,
Jie SONG¹,
Zihan JIANG¹,
Haotian XU¹,
Junxiang ZHU^{1, 2},
Hao WU^{1, 2
, ,}

1.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
2.
Qingdao Special Food Research Institute, Qingdao 266109, China

摘要

摘要: 本文基于层层组装法构建载有β-乳球蛋白纳米颗粒的海藻酸钠-聚乙烯吡咯烷酮-海藻酸钠三层复合膜。通过调节环境pH和温度，同时联合芹菜素配体，诱导β-乳球蛋白形成纳米颗粒。利用粒径、多分散性和Zeta电位表征，研究β-乳球蛋白纳米颗粒的形成规律和贮藏稳定性。然后，将β-乳球蛋白纳米颗粒装载到海藻酸钠-聚乙烯吡咯烷酮-海藻酸钠三层复合膜中，研究纳米载量对膜机械特性、透过特性、光学特性和热特性的影响。结果表明：调整环境pH为7.1，加热温度为75 ℃，在蛋白/配体摩尔比1:8条件下可得到稳定性较好的β-乳球蛋白纳米颗粒。该纳米颗粒以0.2 mg/mL和0.3 mg/mL浓度添加到三层复合膜中时，薄膜的机械特性和水蒸气阻隔性均有明显提升。此外，β-乳球蛋白纳米颗粒的加入还改善了三层复合膜的透光性和热稳定性。综上，装载β-乳球蛋白纳米颗粒的海藻酸钠三层复合膜具有良好的包装特性和应用潜力。
- 海藻酸钠 /
- 聚乙烯吡咯烷酮 /
- β-乳球蛋白 /
- 纳米颗粒 /
- 层层组装 /
- 复合膜
Abstract: In this work, a trilayer composite film of alginate-polyvinylpyrrolidone-alginate loaded with β-lactoglobulin nanoparticles was prepared based on the layer-by-layer assembly. The formation of β-lactoglobulin nanoparticles was induced by adjusting the ambient pH and temperature in combination with binding apigenin. The formation pattern and storage stability of β-lactoglobulin nanoparticles were investigated by particle size, polydispersity and Zeta potential. Then, the β-lactoglobulin nanoparticles were loaded into sodium alginate-polyvinylpyrrolidone-sodium alginate trilayer composite film to investigate the effects of nano-loading on the mechanical, transmission, optical and thermal properties of the film. The results showed that β-lactoglobulin nanoparticles with good stability could be obtained by adjusting the ambient pH to 7.1 and heating the temperature to 75 ℃ under the protein/ligand molar ratio of 1:8. The mechanical properties and water vapor barrier of the films were significantly improved when the nanoparticles were added to the trilayer composite films at concentrations of 0.2 and 0.3 mg/mL. Moreover, the addition of β-lactoglobulin nanoparticles improved the light transmittance and thermal stability of the trilayer composite film. In conclusion, the trilayer composite film made of alginate loaded with β-lactoglobulin nanoparticles exhibited good packaging properties and high application potential.
- alginate /
- polyvinylpyrrolidone /
- β-lactoglobulin /
- nanoparticles /
- layer-by-layer assembly /
- composite film

HTML全文

图 1 pH对β-Lg纳米颗粒粒径、PDI和Zeta电位的影响

Figure 1. Effect of pH on size, PDI, and Zeta potential of β-Lg nanoparticles

注：同一指标不同字母表示差异显著（P<0.05）；图2、图3、图5同。

下载: 全尺寸图片幻灯片

图 2 温度对β-Lg纳米颗粒粒径、PDI和Zeta电位的影响

Figure 2. Effect of temperature on size, PDI, and Zeta potential of β-Lg nanoparticles

下载: 全尺寸图片幻灯片

图 3 β-Lg/芹菜素摩尔比对β-Lg纳米颗粒粒径、PDI和Zeta电位的影响

Figure 3. Effect of molar ratio of β-Lg/apigenin on size, PDI, and Zeta potential of β-Lg nanoparticles

下载: 全尺寸图片幻灯片

图 4 pH（a，b，c）、温度（d，e，f）和摩尔比（g，h，i）对贮藏期内β-Lg纳米颗粒粒径、PDI和Zeta电位的影响

Figure 4. Effect of pH (a, b, c), temperature (d, e, f), and molar ratio (g, h, i) on the size, PDI, and Zeta potential of β-Lg nanoparticles during storage

下载: 全尺寸图片幻灯片

图 5 β-Lg纳米颗粒装载对SA-PVP-SA复合膜水分含量和WVP的影响

Figure 5. Effect of β-Lg nanoparticle loading on moisture content and WVP of SA-PVP-SA composite films

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图 6 β-Lg纳米颗粒装载对SA-PVP-SA复合膜透光率的影响

Figure 6. Effect of β-Lg nanoparticles loading on the light transmission of SA-PVP-SA composite films

下载: 全尺寸图片幻灯片

图 7 不同β-Lg纳米颗粒添加量下三层复合膜的TGA曲线（a）及其一阶导数（b）

Figure 7. The TGA curve (a) and its first-order derivative (b) of trilayer composite films with different adding amount of β-Lg nanoparticles

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表 1 β-Lg纳米颗粒装载对SA-PVP-SA复合膜杨氏模量、拉伸强度、断裂伸长率和韧性的影响

Table 1. Effect of β-Lg nanoparticles loading on Young's modulus, tensile strength, elongation at break, and toughness of SA-PVP-SA composite films

复合膜	杨氏模量（MPa）	拉伸强度（MPa）	断裂伸长率（%）	韧性（MJ/m³）
SA-PVP₀-SA	12.26±0.12^b	47.95±5.15^b	15.03±0.30^b	456.89±2.45^c
SA-PVP_0.1-SA	8.71±0.07^d	48.63±7.55^b	23.77±1.17^a	463.09±8.34^c
SA-PVP_0.2-SA	12.89±0.11^a	52.45±3.65^ab	21.23±3.00^a	663.10±11.35^a
SA-PVP_0.3-SA	9.61±0.08^c	62.48±10.42^a	22.29±2.50^a	625.72±22.12^b
SA-PVP_0.4-SA	7.71±0.07^e	27.25±0.78^c	11.28±0.30^c	189.99±1.12^d
注：同列不同字母表示差异显著（P<0.05）。

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