响应面法优化花青素/Fe<sub>3</sub>O<sub>4</sub>纳米复合物的制备工艺及其体外消化研究

牛芸; 王晴; 韩彬; 彭晓莉

doi:10.13386/j.issn1002-0306.2022080100

响应面法优化花青素/Fe₃O₄纳米复合物的制备工艺及其体外消化研究

doi: 10.13386/j.issn1002-0306.2022080100

成都医学院公共卫生学院，四川成都 610500

基金项目: 四川省科学技术厅省级科技计划项目（2020YJ0169）。

详细信息

作者简介:
牛芸（1998−），女，硕士研究生，研究方向：植物化学物，E-mail：N1037325840@163.com

通讯作者:
彭晓莉（1979−），女，博士，副研究员，研究方向：植物化学物与疾病，E-mail：xiaopeng967@sohu.com

中图分类号: TS201.4
计量
- 文章访问数: 30
- HTML全文浏览量: 26
- PDF下载量: 5
- 被引次数: 0
出版历程
- 收稿日期: 2022-08-10
- 网络出版日期: 2023-05-22
- 刊出日期: 2023-07-01

Optimization of Preparation Process and in Vitro Digestion Study of Anthocyanin/Fe₃O₄ Nanocomposites by Response Surface Methodology

School of Public Healthy, Chengdu Medical College, Chengdu 610500, China

摘要

摘要: 为了提高花青素的生物利用率，本研究采用共沉淀的方法制备花青素/Fe₃O₄纳米复合物。利用响应面法（Response Surface Method，RSM）优化花青素/Fe₃O₄纳米复合物的合成，并对花青素/Fe₃O₄纳米复合物进行粒径分析、Zeta电位测定、扫描电子显微镜、傅里叶变换红外光谱分析以及体外模拟消化实验。结果表明花青素/Fe₃O₄纳米复合物的最佳制备条件为花青素与Fe₃O₄的质量比为1:46，反应时间为19.6 h，反应温度为47 ℃，此工艺条件下花青素的包封率为87.51%。该纳米复合物粒径分布集中在100~1200 nm，且分布均匀，Zeta电位为−48.15 mV。通过扫描电子显微镜观察到花青素与Fe₃O₄纳米粒子间形成了表面光滑的球状颗粒。花青素/Fe₃O₄纳米复合物在1635、1083 cm⁻¹处出现花青素C=O和C-H特征峰。体外消化实验得出花青素在胃液和肠液中的保留率为91.99%和46.23%，DPPH和ABTS⁺自由基清除能力在肠液中均提高（P<0.05）。因此，共沉淀法能够提高花青素的生物利用率，为花青素的高效使用提供了技术支持。
- 花青素/Fe₃O₄纳米复合物 /
- 响应面 /
- 体外消化 /
- 抗氧化活性 /
- 结构表征
Abstract: In order to improve the bioavailability of anthocyanins, a co-precipitation method was used to prepare anthocyanin/Fe₃O₄ nanocomposites in this study. The response surface method was used to optimize the synthesis of anthocyanin/Fe₃O₄ nanocomposites. The particle size analysis, Zeta potential measurement, scanning electron microscope, fourier transform infrared spectroscopy and in vitro digestion simulation of anthocyanin/Fe₃O₄ nanocomposites were carried out. The results showed that the optimum conditions for the preparation of anthocyanin/Fe₃O₄ nnanocomposites were anthocyanin/Fe₃O₄ mass ratio of 1:46, reaction time 19.6 h, reaction temperature 47 ℃. The encapsulation rate of anthocyanin under these conditions was 87.51%. The particle size distribution of anthocyanin/Fe₃O₄ nanocomposites was concentrated in the range of 100~1200 nm with uniform distribution, and the Zeta potential was −48.15 mV. The formation of spherical particles with smooth surfaces between anthocyanin and Fe₃O₄ nanoparticles was observed by scanning electron microscopy. The anthocyanin/Fe₃O₄ nanocomplexes showed anthocyanin C=O and C-H characteristic peaks at 1635 cm⁻¹ and 1083 cm⁻¹. The in vitro digestion simulation showed that the retention of anthocyanin was 91.99% and 46.23% in gastric and intestinal fluids. The scavenging ability of DPPH and ABTS⁺ radicals was increased in the intestinal fluid (P<0.05). Therefore, co-precipitation method can improve the bioavailability of anthocyanins, and provide technical support for the efficient utilization of anthocyanins.
- anthocyanin/Fe₃O₄ nanocomposites /
- response surface methodology /
- in vitro digestion simulation /
- antioxidant activity /
- structure characterization

HTML全文

图 1 矢车菊素-3-O-葡萄糖苷检测波长

Figure 1. Detection wavelength of cyanidin-3-O-glucoside

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图 2 花青素和Fe₃O₄质量比对包封率的影响

Figure 2. Effect of mass ratio of anthocyanin and Fe₃O₄on encapsulation efficiency

注：不同字母表示差异显著（P<0.05）；图3~图4同。

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图 3 花青素和Fe₃O₄反应时间对包封率的影响

Figure 3. Effect of reaction time of anthocyanin and Fe₃O₄ on encapsulation efficiency

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图 4 花青素和Fe₃O₄反应温度对包封率的影响

Figure 4. Effect of reaction temperature of anthocyanin and Fe₃O₄on encapsulation efficiency

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图 5 各因素交互作用对包封率的响应面和等高线图

Figure 5. Response surface plot and contour plot of the effects of each factor on the encapsulation efficiency

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图 6 Fe₃O₄纳米颗粒（a）、花青素/Fe₃O₄纳米复合物（b）的粒径分布

Figure 6. Particle size distributions of Fe₃O₄ (a) and Fe₃O₄/anthocyanin nanocomposites (b)

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图 7 花青素（a）、Fe₃O₄纳米粒子（b）、花青素/Fe₃O₄纳米复合物（c）的微观形貌

Figure 7. SEM images of anthocyanin (a), Fe₃O₄nanoparticles (b), anthocyanin/Fe₃O₄nanocomposites (c)

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图 8 花青素、Fe₃O₄和花青素/Fe₃O₄纳米复合物的傅里叶红外光谱

Figure 8. Fourier transformation infrared spectra of anthocyanin, Fe₃O₄ and anthocyanin/Fe₃O₄ nanocomposites

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图 9 模拟消化对花青素含量的影响

Figure 9. Effects of simulated digestion on anthocyanin content

注：图中同一图例标注不同小写字母，表示在同一消化阶段，不同消化时间具有显著差异（P<0.05）；图10、图11同。

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图 10 模拟消化对纳米复合物DPPH自由基清除能力的影响

Figure 10. Effects of simulated digestion on the DPPH free radical scavenging ability of nanocomposites

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图 11 模拟消化对纳米复合物ABTS⁺自由基清除能力的影响

Figure 11. Effects of simulated digestion on the ABTS⁺ free radical scavenging ability of nanocomposites

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表 1 响应面试验因素水平设计

Table 1. Factors and levels for response surface test

因素		水平
因素	−1	0	1
A质量比	1:40	1:45	1:50
B时间（h）	16	20	24
C温度（℃）	40	50	60

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表 2 响应面试验设计与结果

Table 2. Response surface design experiment conditions and results

试验号	A	B	C	Y（%）
1	1	−1	0	81.89
2	1	0	−1	76.20
3	0	0	0	86.04
4	−1	−1	0	63.81
5	0	1	1	72.17
6	0	0	0	86.17
7	0	1	−1	75.95
8	−1	1	0	79.99
9	0	0	0	88.78
10	0	−1	−1	82.34
11	0	0	0	86.10
12	0	0	0	87.25
13	1	0	1	81.09
14	0	−1	1	63.63
15	−1	0	1	53.66
16	1	1	0	72.23
17	−1	0	−1	81.77

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表 3 Box-Behnken响应面模型的方差分析

Table 3. ANOVA for response surface model of Box-Behnken

来源	平方和	自由度	均方	F	P	显著性
模型	1507.98	9	167.55	47.55	<0.0001	**
A	129.44	1	129.44	36.74	0.0005	**
B	9.40	1	9.40	2.67	0.1465
C	261.18	1	261.18	74.12	<0.0001	**
AB	166.93	1	166.93	47.38	0.0002	**
AC	272.25	1	272.25	77.27	<0.0001	**
BC	55.73	1	55.73	15.82	0.0053	**
A²	170.60	1	170.60	48.42	0.0002	**
B²	152.73	1	152.73	43.35	0.0003	**
C²	225.78	1	225.78	64.08	<0.0001	**
残差	24.66	7	3.52
失拟项	19.10	3	6.37	4.58	0.0879
纯误差	5.56	4	1.39
总离差	1532.65	16
注：P<0.05；*P<0.01。

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表 4 花青素、Fe₃O₄和花青素/Fe₃O₄纳米复合物的Zeta电位

Table 4. Zeta potential of anthocyanin, Fe₃O₄ and anthocyanin/Fe₃O₄ nanocomposites

材料		电位（mV）		平均电位（mV）
花青素	+10.41	+11.02	+11.63	+11.02
Fe₃O₄	−58.18	−58.78	−59.39	−58.78
花青素/Fe₃O₄	−47.55	−48.15	−48.75	−48.15

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