竹笋膳食纤维中结合多酚的提取工艺优化及抗氧化活性分析

冉莎; 张甫生; 李彬; 杨金来; 吴良如; 郑炯

doi:10.13386/j.issn1002-0306.2022090198

竹笋膳食纤维中结合多酚的提取工艺优化及抗氧化活性分析

doi: 10.13386/j.issn1002-0306.2022090198

冉莎^1,,
张甫生¹,
李彬²,
杨金来³,
吴良如^3, ,,
郑炯^1, ,

1.
西南大学食品科学学院，食品科学与工程国家级实验教学示范中心（西南大学），重庆 400715
2.
重庆市林业科学院，重庆 400036
3.
国家林业和草原局竹子研究开发中心，浙江杭州 310012

基金项目: 重庆市科技兴林项目（2021-8；ZD2022-4）；贵州省特色林业产业研发项目（2020-28）；西南大学大学生创新创业训练计划项目（X202210635073）

详细信息

作者简介:
冉莎（2001−），女，本科，研究方向：果蔬加工，E-mail：2030413908@qq.com

通讯作者:
吴良如（1965−），男，本科，研究员，研究方向：竹笋加工，E-mail：bamshoots@163.com

郑炯（1982−），男，博士，副教授，研究方向：果蔬加工，E-mail：zhengjiong_swu@126.com

中图分类号: TS255.36
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- 被引次数: 0
出版历程
- 收稿日期: 2022-09-19
- 网络出版日期: 2023-05-22
- 刊出日期: 2023-07-01

Extraction Optimization and Antioxidant Activity of Bound Polyphenols in Bamboo Shoot Dietary Fiber

Sha RAN^1
,,
Fusheng ZHANG¹,
Bin LI²,
Jinlai YANG³,
Liangru WU^{3
, ,},
Jiong ZHENG^{1
, ,}

1.
College of Food Science, Southwest University, National Demonstration Center for Experimental Food Science and Engineering Education (Southwest University), Chongqing 400715, China
2.
Chongqing Academy of Forestry, Chongqing 400036, China
3.
China National Bamboo Research Center, Hangzhou 310012, China

摘要

摘要: 为探究竹笋膳食纤维（bamboo shoots dietary fiber, BSDF）中结合多酚最佳提取工艺、多酚组成及其抗氧化活性，本研究以BSDF为原料，分别对提取时间、提取温度、碱液浓度和液料比4个因素进行单因素实验，在单因素实验基础上，结合Box-Behnken响应面试验优化BSDF中结合多酚的提取工艺，并对所提取的结合多酚组分进行了初步鉴定，同时以ABTS阳离子自由基、DPPH自由基、超氧阴离子自由基和羟基自由基清除率为指标考察其抗氧化活性。结果表明，BSDF中结合多酚最佳提取条件为碱液浓度9 mol/L、提取温度40 ℃、提取时间4 h、液料比20:1 mL/g，此条件下BSDF中结合多酚提取量预测值为27.95 mg GAE/g BSDF，实际提取量为26.68±0.73 mg GAE/g BSDF，预测准确率95.46%；采用超高效液相色谱-三重四级杆质谱联用仪从多酚提取液中初步鉴定出芥子酸、没食子酸、阿魏酸、香豆酸等12种多酚组分；所得多酚自由基清除率随质量浓度增加呈先升高后逐渐平稳的趋势，在质量浓度为3.0 mg/mL时对ABTS阳离子自由基、超氧阴离子自由基和羟基自由基清除率分别达到73.24%、59.92%和51.41%，浓度为1.25 mg/mL时对DPPH自由基清除率达到76.79%，各组清除率小于同质量浓度抗坏血酸溶液。研究结果可为BSDF中多酚类物质的开发利用提供理论参考。
- 竹笋膳食纤维 /
- 结合多酚 /
- 响应面优化 /
- 组分鉴定 /
- 抗氧化活性
Abstract: To investigate the optimal extraction process, component composition and antioxidant activity of bound polyphenols in bamboo shoots dietary fiber (BSDF), the extraction time, extraction temperature, alkali concentration and liquid-to-material ratio on the extraction yield were researched in this paper. Thereafter, the extraction process of bound polyphenols in BSDF was optimized by Box-Behnken response surface experiment based on single-factor experiments. The components of bound polyphenols were preliminarily identified and the antioxidant activity such as ABTS cation radicals, DPPH radicals, superoxide anion radicals and hydroxyl radicals were investigated. The results showed that alkali concentration of 9 mol/L, extraction temperature of 40 °C, extraction time of 4 h and liquid-to-material ratio of 20:1 mL/g were the optimal condition. Under this condition, the predicted extraction amount of bound polyphenols in BSDF was 27.95 mg GAE/g BSDF while the actual extraction amount was 26.68±0.73 mg GAE/g BSDF, with a prediction accuracy of 95.46%. There were 12 polyphenolic components including sinapic acid, gallic acid, ferulic acid, and coumaric acid were preliminarily identified by ultra performance liquid chromatography-triple quadrupole mass spectrometer. The free radical scavenging rate of polyphenols extract initially increased and then gradually stabilized with the increase of mass concentration. The scavenging rates of ABTS cation radical, superoxide anion radical and hydroxyl radical reached 73.24%, 59.92% and 51.41% with mass concentration of 3.0 mg/mL, respectively. The scavenging rate of DPPH radical reached 76.79% when the concentration was 1.25 mg/mL. The scavenging rate of each group was less than that of ascorbic acid solution of the same mass concentration. The results can provide theoretical reference for the development and utilization of polyphenols in BSDF.
- bamboo shoot dietary fiber /
- bound polyphenols /
- response surface optimization /
- component identification /
- antioxidant activity

HTML全文

图 1 各因素对BSDF中结合多酚提取量的影响

Figure 1. Effects of various factors on the extraction amount of bound polyphenols from BSDF

注：不同小写字母表示各处理间差异显著（P<0.05）。

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图 2 BSDF中结合多酚提取变量及其相互作用的响应面图

Figure 2. Response surface plot of variables extracted with polyphenols and their interactions in BSDF

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图 3 BSDF中结合多酚提取液质谱图

Figure 3. Mass spectrometry of bound polyphenol extract in BSDF

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图 4 BSDF中结合多酚提取液体外抗氧化活性

Figure 4. The antioxidant activity of polyphenols extracted from BSDF in vitro

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表 1 提取BSDF中结合多酚的响应面试验设计因素与水平

Table 1. Response surface test design factors and levels for extraction of bound polyphenols from BSDF

水平	A 提取时间（h）	B 提取温度（°C）	C 碱液浓度（mol/L）	D 液料比（mL/g）
−1	3	35	8	15:1
0	4	40	9	20:1
1	5	45	10	25:1

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表 2 提取BSDF中结合多酚的响应面结果

Table 2. Results of extraction of bound polyphenol response surface from BSDF

试验号	A	B	C	D	多酚提取量（mg GAE/g BSDF）
1	1	1	0	0	23.61±0.89
2	0	0	1	1	21.58±1.37
3	0	1	−1	0	19.03±0.77
4	0	−1	1	0	19.87±0.91
5	0	0	−1	−1	17.14±0.39
6	0	0	0	0	27.19±0.32
7	1	0	0	1	24.02±0.27
8	−1	0	−1	0	19.07±1.03
9	0	−1	0	−1	18.73±0.88
10	0	1	1	0	23.07±0.97
11	0	0	0	0	27.32±0.83
12	1	0	−1	0	19.93±0.58
13	0	−1	−1	0	19.88±0.29
14	0	0	0	0	26.89±0.71
15	0	1	0	−1	20.12±1.07
16	−1	0	0	1	19.08±0.77
17	1	0	1	0	20.18±1.37
18	−1	−1	0	0	18.36±0.97
19	0	0	0	0	28.02±0.81
20	1	−1	0	0	20.93±0.76
21	0	−1	0	1	23.98±0.33
22	1	0	0	−1	18.82±0.45
23	0	0	−1	1	21.76±0.98
24	−1	0	0	−1	17.32±0.79
25	−1	0	1	0	19.82±0.63
26	0	0	0	0	27.83±0.81
27	0	1	0	1	24.76±0.89
28	0	0	1	−1	18.36±0.91
29	−1	1	0	0	20.76±0.68

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表 3 二次响应面模型的方差分析

Table 3. ANOVA for the quadratic response surface model

来源	平方和	自由度	均方	F值	P值	显著性
模型	300.77	14	21.48	28.10	<0.0001	**
A	14.26	1	14.26	18.65	0.0007	**
B	7.68	1	7.68	10.04	0.0068	**
C	3.07	1	3.07	4.02	0.0648
D	50.80	1	50.80	66.44	<0.0001	**
AB	0.0196	1	0.0196	0.0256	0.8751
AC	0.0625	1	0.0625	0.0817	0.7791
AD	2.96	1	2.96	3.87	0.0693
BC	4.10	1	4.10	5.36	0.0362	*
BD	0.0930	1	0.0930	0.1217	0.7324
CD	0.4900	1	0.4900	0.6408	0.4368
A²	99.25	1	99.25	129.80	<0.0001	**
B₂	40.92	1	40.92	53.52	<0.0001	**
C²	113.76	1	113.76	148.78	<0.0001	**
D²	76.78	1	76.78	100.41	<0.0001	**
残差	10.70	14	0.7646
失拟项	9.84	10	0.9837	4.54	0.0791
误差项	0.8674	4	0.2168
总和	311.47	28
注：显著性差异（P<0.05）；*极显著性差异（P<0.01）。

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表 4 最佳条件下提取BSDF中结合多酚的预测值和试验值

Table 4. The predicted and experimental values of binding polyphenols in BSDF extracted under optimal conditions

	提取时间（h）	提取温度（°C）	碱液浓度（mol/L）	液料比（mL/g）	多酚提取量（mg GAE/g BSDF）
预测条件	4.17	40.83	9.07	21.57:1	27.95
实际条件	4	40	9	20:1	26.68±0.73

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表 5 BSDF中结合多酚初步筛选目标物

Table 5. The preliminary screening of target compounds by binding polyphenols in BSDF

编号	离子碎片	绝对强度	相对强度	化合物名称
1	111.25	1770735	14.24	邻苯二酚
2	123.3	681403	5.48	对羟基苯甲醛
3	137.2	156168	15.29	2-羟基苯甲酸
4	140.25	1632401	13.12	香豆酸
5	147.2	489541	47.93	肉桂酸
6	155.2	2703137	21.73	原儿茶酸
7	167.3	675189	5.43	香草酸
8	179.3	79375	7.77	咖啡酸
9	183.25	12437342	100	没食子酸
10	195.2	106819	10.46	阿魏酸
11	198.1	172177	16.86	没食子酸乙酯
12	224.25	1897778	15.26	芥子酸
13	228.4	784631	6.31	白藜芦醇
14	272.3	67057	6.56	柚皮素
15	286.25	62906	6.16	山柰酚
16	288.1	2228613	17.92	儿茶素
17	301.35	66096	6.47	槲皮素
18	305.2	761738	6.12	原儿茶素
19	308.25	298912	29.26	花色苷
20	317.3	76409	7.48	杨梅素
21	335.3	53527	5.24	黄素1
22	355.35	4236422	34.06	绿原酸
23	446.4	2638130	21.21	黄芩苷
24	463.7	59762	5.85	异槲皮素
25	628.5	2066984	16.62	芦丁

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