红蓝草枝和叶提取物不同萃取部位体外抗氧化和抑菌活性研究

黄佳佳; 苏妙仪; 王如意; 吴宏茜; 东方; 李燕杰; 姚玉静

doi:10.13386/j.issn1002-0306.2022100012

红蓝草枝和叶提取物不同萃取部位体外抗氧化和抑菌活性研究

doi: 10.13386/j.issn1002-0306.2022100012

1.
广东食品药品职业学院食品学院，广东广州 510520
2.
广东产品质量监督检验研究院，广东佛山 528300

基金项目: 广东省中医药局科研项目（20202133）；广东省食品检测创新团队项目（2021KCXTD077）；广东食品药品职业学院食品营养与检测教学创新团队项目（2020TD02）；广东食品药品学院科研项目（2021SG03，2021ZR06）；广东食品药品学院大学生创新创业计划训练项目（2021DC02）。

详细信息

作者简介:
黄佳佳（1985−），女，硕士，讲师，研究方向：食品检测，E-mail：313370106@qq.com

通讯作者:
姚玉静（1979−），女，硕士，副教授，研究方向：食品生物技术，E-mail：yaoyj@gdyzy.edu.cn

中图分类号: TS201.2
计量
- 文章访问数: 37
- HTML全文浏览量: 14
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- 被引次数: 0
出版历程
- 收稿日期: 2022-10-08
- 网络出版日期: 2023-05-21
- 刊出日期: 2023-07-01

Antioxidant and Antibacterial Activities of Different Extracted Parts from Branches and Leaves Extracts of the Peristrophe baphica (Spreng.) Bremek

1.
College of Food Science, Guangdong Food and Drug Vocational College, Guangzhou 510520, China
2.
Guangdong Testing Institute for Product Quality Supervision, Foshan 528300, China

摘要

摘要: 以红蓝草枝和叶为原料，探究其乙醇提取物和不同溶剂萃取部位的抗氧化及抑菌活性。采用Folin-Ciocalteu法和NaNO₂-Al(NO₃)₃法分别测定乙醇提取物及各萃取部位总酚和黄酮含量；以清除DPPH•、ABTS⁺•和总还原力为指标评价各部位抗氧化能力并分析抗氧化作用与两种成分之间的相关性。同时，采用牛津杯法测定各部位对常见致病菌的抑菌活性。结果表明，红蓝草枝和叶的乙酸乙酯部位总酚含量最高，分别达79.76 mg/g和80.21 mg/g；枝的氯仿部位和叶的乙醇提取物黄酮含量最高，分别为95.88 mg/g和96.75 mg/g。相关性分析显示红蓝草的抗氧化能力与其酚类含量具有较强相关性。在试验浓度范围内，红蓝草不同组织部位以及不同极性溶剂影响抗氧化及抑菌效果，枝的抗氧化效果优于叶，叶的抑菌效果优于枝。以枝的乙酸乙酯部位，叶的乙酸乙酯和正丁醇部位抗氧化和抑菌效果更为明显，值得进一步研究与开发。
- 红蓝草 /
- 枝 /
- 叶 /
- 提取物 /
- 萃取部位 /
- 抗氧化活性 /
- 抑菌活性
Abstract: In this work, the ethanol extracts of the branches and leaves of Peristrophe baphica (Spreng.) Bremk (PB) as well as the different solvent extracted part of the ethanol extracts were prepared and their antioxidant and antibacterial activities were studied. The contents of total polyphenols and flavonoids of the extracts were determined by Folin-Ciocalteu and NaNO₂-Al(NO₃)₃ method, respectively. The antioxidant activities were evaluated by DPPH radical scavenging ability, ABTS⁺ radical scavenging ability and total reducing power. The relation between the antioxidant activities and the ethanol extracts as well as the different solvent extracted parts was correlated. The antimicrobial activities of the extracts against several common pathogenic bacteria were determined by Oxford cup method. The results showed that the content of total polyphenols in ethyl acetate part were the highest for both branches and leaves, which was 79.76 mg/g and 80.21 mg/g, respectively. The highest content of flavonoids located in chloroform part of branches and the ethanol extract of leaves, which was 95.88 mg/g and 96.75 mg/g, respectively. The correlation analysis showed that the antioxidant activities of each part were strongly correlated with the contents of total polyphenols. In the test concentration range, different parts of tissue and different solvents had important influence on the antioxidant and antimicrobial activities. The antioxidant activities of branches were better than those of leaves, but the antibacterial activities of leaves were better than those of branches. The ethyl acetate part of branches, the ethyl acetate part and the n-butanol part of leaves showed better antioxidant and antimicrobial activities and could be used for further application.
- Peristrophe baphica (Spreng.) Bremk /
- branch /
- leaf /
- extracts /
- extracted part /
- antioxidant activity /
- antibacterial activity

HTML全文

图 1 红蓝草枝和叶提取物不同萃取部位总酚含量（n=3）

Figure 1. Total phenol content in different extracted parts of PB branches and leaves extracts (n=3)

注：不同小写字母代表枝的不同溶剂萃取部位含量差异显著（P<0.05），不同大写字母代表叶的不同溶剂萃取部位含量差异显著（P<0.05）；*表示同一溶剂不同组织部位含量差异显著（P<0.05），**表示同一溶剂不同组织部位含量差异极显著（P<0.01）；图2同。

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图 2 红蓝草枝和叶提取物不同萃取部位黄酮含量（n=3）

Figure 2. The content of total flavonoids in different extracted parts of PB branches and leaves extracts (n=3)

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图 3 红蓝草枝（a）和叶（b）提取物不同萃取部位对DPPH•清除作用（n=3）

Figure 3. Scavenging effect of different extracted parts of PB branches (a) and leaves (b) extracts on DPPH free radicals (n=3)

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图 4 红蓝草枝（a）和叶（b）提取物不同萃取部位对ABTS⁺•清除作用（n=3）

Figure 4. Scavenging effect of different extracted parts of PB branches (a) and leaves (b) extracts on ABTS⁺ free radicals (n=3)

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图 5 红蓝草枝（a）和叶（b）提取物不同萃取部位总还原力（n=3）

Figure 5. Total reducing power of different extracted parts of PB branches (a) and leaves (b) extracts (n=3)

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表 1 体外抗氧化活性与成分含量相关系数

Table 1. Correlation coefficient between antioxidant activity in vitro and component content

体外抗氧化指标	枝		叶
体外抗氧化指标	总酚	黄酮	总酚	黄酮
DPPH•清除率	0.968^**	0.569	0.696	−0.174
ABTS⁺•清除率	0.684	0.147	0.600	−0.312
总还原力	0.970^**	0.342	0.639	−0.663
注：**双侧极显著相关（P<0.01）。

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表 2 红蓝草枝和叶提取物对不同菌株的抑制作用（n=3）

Table 2. The inhibition effect of different extracted parts of PB branches and leaves extracts against different strains (n=3)

试验菌株	样品质量浓度（mg/mL）	抑菌直径（mm）
		醇提物		石油醚部位	氯仿部位		乙酸乙酯部位		正丁醇部位		水相部位
		枝	叶	叶	枝	叶	枝	叶	枝	叶	枝
金黄色葡萄球菌（G⁺）	62.5	−	−	11.86±0.22^a	−	−	10.68±0.34^c	11.22±0.62^b	−	10.45±0.09^c	9.21±0.20^d
	125	−	11.23±0.44^e	13.32±0.85^bc	9.04±0.32^g	12.11±0.50^d	14.02±0.55^b	15.75±0.28^a	−	12.52±0.14^cd	10.13±0.20^f
	250	13.19±0.30^d	13.79±0.39^d	15.34±0.78^c	10.18±0.22^f	13.27±0.42^d	16.83±0.35^b	18.75±0.63^a	−	16.22±0.51^bc	12.15±0.29^e
大肠埃希氏菌（G⁻）	62.5	−	−	−	−	−	13.18±0.38^a	−	−	−	−
	125	−	−	−	10.39±0.11^b	−	16.18±0.42^a	−	−	−	−
	250	−	11.86±0.48^bc	−	12.39±0.73^b	−	18.43±0.14^a	−	−	11.41±0.13^c	−
蜡样芽胞杆菌（G⁺）	62.5	−	−	−	−	−	−	−	−	9.49±0.20^a	−
	125	−	10.00±0.42^c	−	11.18±0.16^b	−	−	−	−	12.51±0.66^a	−
	250	12.86±0.33^b	13.92±0.51^a	−	13.05±0.34^b	−	11.91±0.41^c	11.16±0.45^d	−	14.23±0.69^a	−
奇异变形杆菌（G⁻）	62.5	−	9.51±0.26^b	−	−	−	−	−	−	10.70±0.32^a	−
	125	9.44±0.31^d	13.43±0.39^b	9.23±0.33^d	−	−	−	12.09±0.18^c	−	13.99±0.16^a	−
	250	10.99±0.28^d	16.99±0.50^a	12.89±0.27^c	−	−	−	15.78±0.48^b	−	17.15±0.25^a	10.89±0.31^d
铜绿假单胞菌（G⁻）	62.5	−	−	−	−	−	−	−	−	9.10±0.53^a	−
	125	−	−	−	−	−	−	−	−	10.44±0.56^a	−
	250	10.98±0.24^b	11.10±0.68^b	−	−	−	−	−	−	12.48±0.76^a	−
白色念珠菌（真菌）	62.5	−	−	−	−	−	−	−	−	−	−
	125	9.87±0.33^a	9.25±0.20^b	−	−	−	−	−	−	−	−
	250	13.29±0.64^a	11.27±0.46^b	−	−	−	−	−	13.23±0.47^a	−	−
阪崎肠杆菌（G⁻）	62.5	−	−	−	−	−	−	−	−	−	−
	125	−	9.79±0.41^a	−	−	−	−	−	−	−	−
	250	−	13.08±0.60^a	−	−	−	−	−	−	−	−
肺炎克雷伯氏菌（G⁻）	62.5	−	−	−	−	−	−	−	−	−	−
	125	−	−	−	−	−	−	−	−	−	−
	250	−	−	−	−	−	−	−	−	11.99±0.17^a	11.23±0.34^b
蒸馏水		−	−	−	−	−	−	−	−	−	−
注：牛津杯直径8 mm；“-”为未见抑菌圈；G⁺为革兰氏阳性菌，G⁻为革兰氏阴性菌。含不同小写字母代表不同部位在相同质量浓度对同一致病菌抑菌圈大小差异显著（P<0.05）。

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