茉莉酸甲酯处理对鲜切甜瓜贮藏期间苯丙烷代谢的影响

李如新; 郭媛媛; 解春艳; 贾云; 张兵兵; 罗海波; 武张飞

doi:10.13386/j.issn1002-0306.2022100172

茉莉酸甲酯处理对鲜切甜瓜贮藏期间苯丙烷代谢的影响

doi: 10.13386/j.issn1002-0306.2022100172

李如新^1,,
郭媛媛^{1, 2},
解春艳^{1, 2, 3},
贾云¹,
张兵兵⁴,
罗海波⁵,
武张飞^{1, 2, 3, ,}

1.
廊坊师范学院生命科学学院，河北廊坊 065000
2.
河北省食药用菌资源高值利用技术创新中心，河北廊坊 065000
3.
廊坊市食品营养与安全重点实验室，河北廊坊 065000
4.
中国人民警察大学教学保障处，河北廊坊 065000
5.
南京师范大学食品与制药工程学院，江苏南京 210023

基金项目: 河北省重点研发计划项目（19227133D）；河北省自然科学基金青年科学基金项目（C2022408017）；河北省高等学校科学技术研究项目（QN2022174）；新疆生产建设兵团第一师阿拉尔市科技计划项目（2022XX05）；国家级大学生创新创业训练计划项目（202210100002）；廊坊师范学院博士科研启动项目（XBQ202141）。

详细信息

作者简介:
李如新（2002−），女，本科，研究方向：果蔬加工与贮藏，E-mail：2978666939@qq.com

通讯作者:
武张飞（1990−），男，博士，讲师，研究方向：果蔬加工与贮藏，E-mail：wuzhangfeidsh@126.com

中图分类号: TS255.3
计量
- 文章访问数: 23
- HTML全文浏览量: 11
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2022-10-19
- 网络出版日期: 2023-06-19

Effect of Methyl Jasmonate Treatment on Phenylpropanoid Pathway in Fresh-cut Melon during Storage

LI Ruxin^1
,,
GUO Yuanyuan^{1, 2},
XIE Chunyan^{1, 2, 3},
JIA Yun¹,
ZHANG Bingbing⁴,
LUO Haibo⁵,
WU Zhangfei^{1, 2, 3
, ,}

1.
College of Life Science, Langfang Normal University, Langfang 065000, China
2.
Technology Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang 065000, China
3.
Langfang Key Laboratory of Food Nutrition and Safety, Langfang 065000, China
4.
Teaching Guarantee Office, China People's Police University, Langfang 065000, China
5.
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China

摘要

摘要: 茉莉酸甲酯（methyl jasmonate，MeJA）是一种天然存在的内源物质和信号分子，在植物胁迫应激反应和生长发育过程中起到调节作用。以“西州蜜-17”甜瓜为试材，采用100 μmol/L MeJA 20 ℃熏蒸20 h，研究其对鲜切甜瓜贮藏期间总酚含量和苯丙烷代谢途径关键酶活性和基因表达水平的影响。结果表明：切分前MeJA处理能够显著提高贮藏期间鲜切甜瓜总酚含量，增加苯丙氨酸解氨酶（phenylalanine ammonia-lyase, PAL）、肉桂酸4-羟化酶（cinnamate 4-hydroxylase，C4H）和4-香豆酸-CoA连接酶（4-coumarate: CoA ligase，4CL）活性，同时还发现MeJA处理提高了贮藏期间鲜切甜瓜CmPAL1/2/3、CmPAL5-9、CmC4H1/2/4和Cm4CL1/2的表达量（P<0.05）。由此表明，切分前MeJA处理能提高贮藏期间鲜切甜瓜苯丙烷代谢的水平，诱导酚类物质的积累。
- 鲜切 /
- 甜瓜 /
- 茉莉酸甲酯 /
- 苯丙烷代谢通路 /
- 酚类物质
Abstract: Methyl jasmonate (MeJA), as a natural endogenous substance and a signal molecule, plays an important role in stress response and development in plant. The effect of MeJA fumigation (100 μmol/L, treatment at 20 ℃ for 20 h) on phenylpropanoid pathway during storage of 'Xizhoumi-17' melon was assayed in this study. Results showed that MeJA treatment significantly increased the total phenolics content, and enhanced the activities of phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H) and 4-coumarate: CoA ligase (4CL) (P<0.05). In addition, the expression of CmPAL1/2/3, CmPAL5-9, CmC4H1/2/4, and Cm4CL1/2 was also upregulated by MeJA treatment (P<0.05). Taken together, these results imply that MeJA treatment of fresh-cut melon would elevate the phenylpropanoid pathway and lead to the accumulation of phenolics.
- fresh-cut /
- melon /
- methyl jasmonate /
- phenylpropanoid metabolic pathway /
- phenolic compounds

HTML全文

图 1 MeJA处理对贮藏期间鲜切甜瓜总酚含量的影响

Figure 1. Effect of methyl jasmonate treatment on the content of total phenolic compounds in fresh-cut melon during storage

注：图中不同小写字母表示相同贮藏时间MeJA处理与对照组差异显著（P<0.05）；图2~图4同。

下载: 全尺寸图片幻灯片

图 3 MeJA处理对贮藏期间鲜切甜瓜PAL基因表达量的影响

Figure 3. Effect of methyl jasmonate treatment on the gene expression level of phenylalanine ammonia-lyase in fresh-cut melon during storage

下载: 全尺寸图片幻灯片

图 4 MeJA处理对贮藏期间鲜切甜瓜C4H和4CL基因表达量的影响

Figure 4. Effect of methyl jasmonate treatment on the gene expression level of cinnamate 4-hydroxylase and 4-coumarate: CoA ligase in fresh-cut melon during storage

下载: 全尺寸图片幻灯片

图 2 MeJA处理对贮藏期间鲜切甜瓜PAL、C4H和4CL活性的影响

Figure 2. Effect of methyl jasmonate treatment on the activity of phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H) and 4-coumarate: CoA ligase (4CL) in fresh-cut melon during storage

下载: 全尺寸图片幻灯片

表 1 鲜切甜瓜苯丙烷代谢关键基因qRT-PCR引物序列

Table 1. Primer sequence of genes involved in phenylpropanoid pathway for qRT-PCR in fresh-cut melon

基因名称	基因编号	上游引物	下游引物	退火温度（℃）
CmPAL1	MELO3C014223	AGGAACAAGGCTTTGCATGG	GCTCGGGTTTCTACTTGCAG	56.1
CmPAL2	MELO3C014224	GCTGAGGCTGCCTTTAAACA	CCTACAGCAGTGCCATTGAC	56.0
CmPAL3	MELO3C014226	GGGAAGCTCATGTTTGCTCA	TGCTCAGCACTTTGAACATGA	55.5
CmPAL4	MELO3C014227	CTGCAAGCAGAAACCCAAGT	GTTGCTCAGCACTTTGGACA	56.1
CmPAL5	MELO3C014228	CTGCAAGCAGAAACCCAAGT	GTTGCTCAGCACTTTGGACA	56.1
CmPAL6	MELO3C014229	GCAGAGGGAGCTCATACGAT	GTAGCCTTGAAGGAGGGTGT	56.1
CmPAL7	MELO3C017809	CTGGTGAAGCTTGGAGGAGA	AGTGCCTTTACCCATGCTCT	56.0
CmPAL8	MELO3C017810	AGAGCATGGGTAAAGGCACT	TTGTTGCGGAATGAGGCAAA	56.0
CmPAL9	MELO3C017811	CTGGTGAAGCTTGGAGGAGA	AGTGCCTTTACCCATGCTCT	56.0
CmC4H1	MELO3C003932	GCCAAGTTCTCCATGCTCAG	CATCCTCCCACATGCCACTA	56.2
CmC4H2	MELO3C019585	CAGAGCTAGTGAACCACCCA	GGAGGTATGGGAGCTTGTGT	56.1
CmC4H3	MELO3C003933	GCTTGCTAGCCAAGTTCTCC	TCTACGCATCTTACGCCAGT	56.0
CmC4H4	MELO3C003934	GGAATTCAGGCCAGAGAGGT	TTCTTCTTCCAACGCCGAAC	56.1
Cm4CL1	MELO3C023493	TCCCGACATTCACATTCCCA	CCGTAAGCTGAACGTCATGG	56.2
Cm4CL2	MELO3C024886	ATGAAGATCGTCGACACCGA	TGCCTCCGGATTGTTGAGAT	56.1
β-actin	MELO3C023264	CCGTTCTGTCCCTCTATGCT	AGTAAGGTCACGACCAGCAA	56.0

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