响应面法优化丁酸梭菌发酵培养工艺

邢宏观; 林建国; 钟雪兆; 王常高; 杜馨; 蔡俊

doi:10.13386/j.issn1002-0306.2016.19.038

响应面法优化丁酸梭菌发酵培养工艺

Optimization of fermentation process for Clostridium butyricum using response surface methodology

摘要

摘要: 采用响应面法对丁酸梭菌生物量的发酵工艺进行优化。在单因素实验的基础上利用Plackett-Burman实验设计筛选出影响菌体数的显著性因素。在此基础上,运用最陡爬坡实验找出CCD实验的中心点,并确定非显著因素最低添加量来降低生产成本。最后通过响应面法分析获得最佳发酵培养基组成成份。结果表明:酵母浸粉、Fe SO4和K2HPO4为影响菌体量的3个显著性因素;可溶性淀粉2%、酵母浸粉6%、Fe SO41.74%、K2HPO40.37%、Na Cl 0.2%、Mg SO40.024%为最佳培养基组合;优化后的菌体数可达1.01×109个/m L,与优化前（2.3×108个/m L）相比,提高至4.39倍。

Abstract: Response surface methodology( RSM) was employed to optimize fermentation process for improving the biomass of Clostridium butyricumm.On the basis of single factor experiment,factors which influenced the biomass of Clostridium butyricumm significantly were screened by Plackett- Burman design. On this basis,the steepest ascent experiment was applied to find out the center point of central composite design and the minimum amount experiment was used to reduce the cost of non- significant factors. Finally,the maximum response value was identified by CCD. The results showed that: Yeast extract powder,FeSO_4 and K_2HPO_4 were the three factors influencing the biomass of C.Butyricumm significantly,soluble starch 2%,yeast extract powder 6%,FeSO_4 1.74%,K_2HPO_4 0.37%,Na Cl 0.2% and MgSO_4 0.024% were the best combination of medium component,the biomass of Clostridium butyricum reached 1.01 × 109 cells / m L which was 4.39 times that of the biomass before optimization.