Abstract: In order to satisfy the demand of consumers for a diversified intake of high quality protein, a fermented plant-based milk substitute product was developed from pea protein. The relationship between the addition of pea protein powder, pea starch and white sugar and the organoleptic quality of the product was analysed in an orthogonal test to determine the optimum amount of pea protein powder, pea starch and white sugar. The results showed that the best fermentation effect was achieved at 4 h and 10% inoculum of fermenting agent. The main physical properties of the product were as follows: Brightness (L^*) 74.74±0.02, hardness 124.45±0.30 g, viscosity 233.11±7.27 g·s, water holding capacity 92.13%. The product was a non-Newtonian fluid and elastic, the surface microstructure was denser, more porous and smaller compared to traditional yoghurt. Therefore, this study provides technical support for the development of new plant-based yoghurt and further broadening the application and production of plant-based yogurt.