负载儿茶素的聚乙烯醇/壳聚糖电纺膜的构建及性能分析

方超逸; 肖菲菲; 陈宇桦; 邓仕彬

doi:10.13386/j.issn1002-0306.2023070207

负载儿茶素的聚乙烯醇/壳聚糖电纺膜的构建及性能分析

Construction and Properties of Polyvinyl Alcohol/Chitosan Electrospun Film Loaded with Catechins

摘要

摘要: 为开发新型生物基食品包装材料，提高天然酚类化合物的生物利用度，本文以儿茶素（Catechin，CT）、聚乙烯醇（Polyvinyl alcohol，PVA）和壳聚糖（Chitosan，CS）为基材，通过静电纺丝法制备负载儿茶素的电纺膜。利用扫描电镜分析电纺膜的微观形貌与直径分布；通过傅里叶红外光谱、X射线衍射、热分析等表征技术探讨CT与成膜基材之间的相互作用；以电纺膜的机械性能、气体透过率及抗氧化活性为参数，考察CT的添加量对电纺膜理化性能的影响；最后将电纺膜应用于草莓保鲜研究，通过感官评价并结合失水率、可滴定酸含量等指标分析电纺膜的保鲜效果。结果表明，CT与CS之间相互作用形成分子间氢键，疏水性能提高；当CT浓度为0.8%时，电纺膜的综合性能最佳：其形貌良好、结构致密，具有较好的热稳定性；此时其拉伸强度和断裂伸长率均达到最大值，分别为12.89 MPa和62.45%；其水溶性、水蒸气透过率和CO₂透过率均最低，分别为29.51%、0.1532 g·mm·(m²·h·kPa)⁻¹和5.9 g·(m²·h)⁻¹；此时DPPH自由基清除率也达到最大值71.02%；并且电纺膜存在缓释作用，当CT浓度为0.8% 时，其累积释放率最高；保鲜研究结果表明，电纺膜能有效延缓草莓的腐烂变质，并且0.8% CT浓度的电纺膜其保鲜效果最佳。综上，0.8% CT浓度的电纺膜，其综合性能最佳，具有一定的抗氧化性和保鲜效果。

Abstract: In order to develop a new bio-based food packaging material and improve the bioavailability of natural phenolic compounds, an electrospinning film loaded with Catechin was prepared by electrospinning using catechin (CT), Polyvinyl alcohol (PVA) and Chitosan (CS) as substrates. The microstructure and diameter distribution of the electrospun films were analyzed by scanning electron microscopy. The interaction between CT and film forming substrate was studied by Fourier infrared spectroscopy, X-ray diffraction and thermal analysis. The effects of CT addition on the physical and chemical properties of the electrospun film were investigated by taking the mechanical properties, gas permeability and antioxidant activity of the film as parameters. Finally, the preservation effect of electrospun film was analyzed by sensory evaluation, water loss rate and titrable acid content. The results showed that the intermolecular hydrogen bond was formed between CT and CS, and the hydrophobic property was improved. When the concentration of CT was 0.8%, the comprehensive performance of the electrospun film was the best. It had good morphology, dense structure and good thermal stability. At this point, the tensile strength and elongation at break reached the maximum, which were 12.89 MPa and 62.45% respectively. The water solubility, water vapor transmittance and CO₂ transmittance were the lowest, which were 29.51%, 0.1532 g·mm·(m²·h·kPa)⁻¹ and 5.9 g·(m²·h)⁻¹, respectively. Besides, the scavenging rate of DPPH free radical also reached the maximum value, which was 71.02%. Moreover, the electrospun membrane had sustained release effect. When the CT concentration was 0.8%, its cumulative release rate was the highest. The preservation research results showed that the electrospun film could effectively delay the deterioration of strawberry, and the electrospun film with 0.8% CT concentration had the best preservation effect. In conclusion, the electrospun film with 0.8% CT concentration had the best comprehensive performance, and it had certain antioxidation and fresh-keeping ability.