表没食子儿茶素没食子酸酯对葡聚糖硫酸钠诱导的小鼠结肠炎的改善作用

李杰; 张志旭

doi:10.13386/j.issn1002-0306.2022080331

表没食子儿茶素没食子酸酯对葡聚糖硫酸钠诱导的小鼠结肠炎的改善作用

doi: 10.13386/j.issn1002-0306.2022080331

李杰^1,,
张志旭^{2, 3, 4, ,}

1.
湖南农业大学食品科学技术学院，湖南长沙 410128
2.
湖南农业大学园艺学院，湖南长沙 410128
3.
国家中医药管理局亚健康干预技术实验室，湖南长沙 410128
4.
湖南植物功能成分利用协同创新中心，湖南长沙 410128

基金项目: 湖南省教育厅科学研究项目优秀青年项目（20B282）；湖南省自然科学基金联合基金（2022JJ5007）；湖南省自然科学基金（2023JJ50004）。

详细信息

作者简介:
李杰（1987−），男，硕士，助教，研究方向：植物提取物功能成分利用，E-mail：lijie@hunau.edu.cn

通讯作者:
张志旭（1972−），男，博士，副教授，研究方向：品质分析及功能产品筛选评价，E-mail：zhang_zhixu@hunau.edu.cn

中图分类号: TS201.4
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出版历程
- 收稿日期: 2022-08-31
- 网络出版日期: 2023-05-22
- 刊出日期: 2023-07-01

Improving Effects of Epigallocatechin-3-gallate (EGCG) on Dextran Sulfate Sodium (DSS)-Induced Colitis

Jie LI^1
,,
Zhixu ZHANG^{2, 3, 4
, ,}

1.
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128 , China
2.
Horticulture College, Hunan Agricultural University, Changsha 410128, China
3.
State Key Laboratory of Subhealth Intervention Technology, Changsha 410128, China
4.
Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, China

摘要

摘要: 本研究旨在探讨表没食子儿茶素没食子酸酯（Epigallocatechin-3-gallate，EGCG）对葡聚糖硫酸钠（Dextran sulfate sodium，DSS）诱导的小鼠结肠炎和肠道菌群的影响。将C57BL/6雄性小鼠分为正常对照组、肠炎模型组和EGCG处理组（50 mg/kg），每组10只，连续灌胃给药9 d。通过称取小鼠体质量，观察并记录小鼠大便黏稠度、大便出血情况，测量小鼠结肠长度和检测血清中炎症因子来评估EGCG对DSS诱导的小鼠结肠炎症的改善作用；通过分析结肠病理形态、紧密连接蛋白的表达量、肠道菌群多样性和肠道菌群结构来评估EGCG对DSS诱导的结肠炎小鼠肠道菌群的影响。结果表明，EGCG能够有效改善DSS诱导的结肠炎小鼠体质量的下降、腹泻、便血、结肠缩短等不良反应；缓解DSS诱导的小鼠结肠炎导致的全身性慢性炎症和肠道屏障损伤；改善DSS诱导的结肠炎小鼠肠道菌群紊乱，恢复肠道菌群多样性，降低厚壁菌门的相对丰度，提高拟杆菌门的相对丰度，促进有益菌Akkermansia、Alistipes和Bacteroides的增殖并抑制有害菌Desulfovibrio、Escherichia-Shigella和Helicobacter的生长。因此，EGCG通过保护肠道屏障和调节肠道菌群紊乱，从而有效改善DSS诱导的小鼠结肠炎症。
- 表没食子儿茶素没食子酸酯 /
- 葡聚糖硫酸钠 /
- 结肠炎 /
- 肠道菌群
Abstract: This study aimed to investigate the effect of epigallocatechin-3-gallate (EGCG) on dextran sulfate sodium (DSS)-induced colitis and gut microbiota in mice. The C57BL/6 male mice were divided into normal control group, enteritis model group and EGCG treatment group (50 mg/kg), 10 mice in each group, and were administered orally for 9 consecutive days. The improvement effect of EGCG on DSS-induced colitis in mice was evaluated by weighing the weight of the mice, observing and recording the fecal viscosity and fecal bleeding of the mice, measuring the length of the colon in the mice and detecting inflammatory factors in the serum. The effect of EGCG on the gut microbiota of DSS-induced colitis mice was evaluated by analyzing colon pathological morphology, expression of tight junction proteins, the diversity and structure of gut microbiota. The results showed that EGCG effectively improved the adverse reactions in DSS-induced colitis mice such as weight loss, diarrhea, blood in the stool, and colon, and alleviated systemic chronic inflammation and intestinal barrier damage in DSS-induced colitis mice. Furthermore, EGCG also ameliorated the gut microbiota disturbance, restored gut microbiota diversity, decreased the relative abundance of Firmicutes, increased the relative abundance of Bacteroidetes, promoted the proliferation of beneficial bacteria such as Akkermansia, Alistipes and Bacteroides, and inhibited the growth of harmful bacteria including Desulfovibrio, Escherichia-Shigella and Helicobacter. Therefore, EGCG effectively ameliorated DSS-induced colitis in mice by protecting the intestinal barrier and regulating gut microbiota disturbance.
- epigallocatechin-3-gallate /
- dextran sulfate sodium /
- colitis /
- gut microbiota

HTML全文

图 1 EGCG对DSS诱导的结肠炎小鼠体质量（A）、疾病活动指数（B）和结肠长度（C）的影响

Figure 1. The effect of EGCG on body weight (A), disease activity index (B) and colon length (C) of DSS-induced colitis mice

注：不同字母表示不同处理组差异显著（P<0.05），图2、图4~图7同。

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图 2 EGCG对DSS诱导的结肠炎小鼠血清中IL-1β、IL-6和TNF-α的影响

Figure 2. The effect of EGCG on serum IL-1β, IL-6 and TNF-α in DSS-induced colitis mice

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图 3 各组小鼠结肠组织H&E和PAS染色（200×）

Figure 3. H&E and PAS staining of colon tissue of mice in each group (200×)

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图 4 EGCG对DSS诱导的结肠炎小鼠肠道屏障蛋白ZO-1的影响

Figure 4. The effect of EGCG on the intestinal barrier protein ZO-1 in DSS-induced colitis mice

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图 5 EGCG对DSS诱导的结肠炎小鼠肠道菌群多样性的影响

Figure 5. The effect of EGCG on the diversity of intestinal flora in DSS-induced colitis mice

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图 6 EGCG对DSS诱导的结肠炎小鼠肠道菌群门水平的影响

Figure 6. The effect of EGCG on the phylum level of intestinal flora in DSS-induced colitis mice

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7 EGCG对DSS诱导的结肠炎小鼠肠道菌群属水平的影响

7. The effect of EGCG on the level of intestinal flora in DSS-induced colitis mice

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表 1 疾病活动指数评价标准

Table 1. Scoring criteria of disease activity index

大便黏稠度	大便出血	体质量下降百分率（%）	得分
正常	正常	不变	0
轻度软便	潜血弱阳性	1~5	1
严重软便	潜血阳性	6~10	2
轻度腹泻	潜血强阳性	11~15	3
严重腹泻	显性出血	>15	4

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