QuEChERS EMR Lipid净化结合同位素稀释-超高效液相色谱-串联质谱法同时测定畜肉中17种<i>β</i>-受体激动剂

毛锐; 林浩; 刘川; 姚静; 肖全伟; 戴琴

doi:10.13386/j.issn1002-0306.2022090195

QuEChERS EMR Lipid净化结合同位素稀释-超高效液相色谱-串联质谱法同时测定畜肉中17种β-受体激动剂

doi: 10.13386/j.issn1002-0306.2022090195

成都市食品检验研究院，国家市场监管重点实验室（营养与健康化学计量及应用），四川成都 611130

详细信息

作者简介:
毛锐（1989−），女，硕士，工程师，研究方向：食品安全与质量检测，E-mail：maorui0428@126.com

通讯作者:
戴琴（1984−），女，硕士，高级工程师，研究方向：食品安全与质量检测，E-mail：740747208@qq.com

中图分类号: O657.63
计量
- 文章访问数: 30
- HTML全文浏览量: 10
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2022-09-20
- 网络出版日期: 2023-06-19

Simultaneous Determination of 17 β-Receptor Agonists in Meat by QuEChERS EMR-Lipid with Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Chengdu Institute of Food Inspection, Chengdu 611130, China

摘要

摘要: 目的：建立了一种采用QuEChERS EMR Lipid净化结合同位素稀释-超高效液相色谱-串联质谱技术同时测定畜肉中17种β-受体激动剂药物残留的检测方法。方法：样品加入pH为5.2的乙酸铵缓冲溶液，以β-葡萄糖醛苷酶/芳基硫酸酯酶酶解，经5%甲酸乙腈提取，QuEChERS EMR Lipid净化，采用CORTECS™ UPLC^® C₁₈柱（3.0 mm×100 mm，1.6 μm），0.1%甲酸水和甲醇梯度洗脱，在分时段多反应监测（scheduled MRM，sMRM）模式下测定，内标法定量。结果：17种β-受体激动剂在0~20 ng/mL浓度范围内线性相关系数均大于0.99，方法检出限为0.01~0.09 μg/kg，定量限为0.05~0.31 μg/kg。在0.5、2.0、5.0 μg/kg 3个加标浓度水平下的平均回收率为81.7%~111.8%，RSD为0.8%~10.3%（n=6）。100批市售生鲜畜肉中未检出β-受体激动剂。结论：该法前处理步骤简便，净化效果良好，缩短酶解时间，提高了样品检测效率，内标法定量精准可靠，适用于大批量畜肉样品中多种β-受体激动剂药物残留的同时检测。
- β-受体激动剂 /
- 畜肉 /
- 超高效液相色谱-串联质谱法（UPLC-MS/MS） /
- QuEChERS /
- 同位素内标
Abstract: Objective: A method for simultaneous determination of 17 β-receptor agonists in meat was established by QuEChERS EMR-Lipid with isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry. Methods: The samples were added to ammonium acetate buffer solution (pH5.2) and enzymatic hydrolyzed by β-glucuronidase and arysulfatase. The hydrolysates were extracted by acetonitrile containing 5% formic acid, and purified by QuEChERS EMR-Lipid. The analyses were separated by CORTECS™ UPLC^® C₁₈ chromatographic column with gradient elution using 0.1% formic acid and methanol as the mobile phase. The target compounds were monitored in scheduled MRM mode. Then the internal standard method was used for quantitative analysis. Results: The linear regression correlation coefficients for the 17 β-receptor agonists were all higher than 0.99 in the range from 0 ng/mL to 20 ng/mL. The limits of detection were 0.01~0.09 μg/kg and the limits of quantification were 0.05~0.31 μg/kg. The average recoveries at three spiked levels of 0.5, 2.0 and 5.0 μg/kg ranged from 81.7% to 111.8% with the relative standard deviations of 0.8%~10.3% (n=6). No β-receptor agonists were detected in 100 batches of fresh meat. Conclusion: The method has the advantages of simple pretreatment steps and good purification effect. It shortened enzymolysis time, improved sample detection efficiency. The internal standard method was accurate and reliable. It was suitable for simultaneous determination of β-receptor agonists in a large number of meat samples.
- β-receptor agonists /
- meat /
- ultra performance liquid chromatograph-tandem mass spectrometry (UPLC-MS/MS) /
- QuEChERS /
- isotope internal standard

HTML全文

图 1 17种β-受体激动剂的提取离子流色谱图

Figure 1. Extracted ion chromatogram of 17 β-receptor agonists

注：1.特布他林；2.沙丁胺醇；3.西布特罗；4.非诺特罗；5.羟甲基克伦特罗；6.莱克多巴胺；7.氯丙那林；8.克伦特罗；9.福莫特罗；10.妥布特罗；11.溴布特罗；12.马布特罗；13.克仑潘特；14.班布特罗；15.马喷特罗；16.苯乙醇胺A；17.喷布特罗。

下载: 全尺寸图片幻灯片

图 2 牛肉、猪肉、羊肉样品中17种β-受体激动剂的绝对萃取回收率

Figure 2. Absolute extraction recovery of 17 β-receptor agonists in beef, pork and mutton

下载: 全尺寸图片幻灯片

图 3 牛肉、猪肉、羊肉样品中17种β-受体激动剂的基质效应

Figure 3. Matrix effect of 17 β-receptor agonists in beef, pork and mutton

下载: 全尺寸图片幻灯片

图 4 牛肉样品QuEChERS法与MCX法基质效应对比图

Figure 4. Matrix effect comparison between QuEChERS method and MCX method in beef samples

下载: 全尺寸图片幻灯片

图 5 牛肉样品QuEChERS法与MCX法绝对萃取回收率对比图

Figure 5. Absolute extraction recovery comparison between QuEChERS method and MCX method in beef samples

下载: 全尺寸图片幻灯片

表 1 17种β-受体激动剂的质谱参数

Table 1. Mass spectrometric parameters of 17 β-receptor agonists

分析物	内标	保留时间（min）	监测离子（m/z）	去簇电压（V）	碰撞能量（eV）
羟甲基克伦特罗	克伦特罗-D₉	7.25	293.1>203.1*，293.1>275.1	65	27，17
马喷特罗	马布特罗-D₉	12.26	324.8>237.1*，324.8>217.1	65	25，37
喷布特罗	喷布特罗-D₉	14.49	292.3>236.2*，292.3>201.1	65	22，28
克伦特罗	克伦特罗-D₉	9.48	277.1>203.1*，277.1>259.1	65	22，16
特布他林	特布他林-D₉	3.43	226.1>152.1*，226.1>125.1	65	23，32
溴布特罗	溴布特罗-D₉	11.05	367.0>293.0*，367.0>348.9	65	26，17
苯乙醇胺A	苯乙醇胺A-D₃	12.74	345.2>150.2*，345.2>327.2	65	33，19
非诺特罗	莱克多巴胺-D₃	4.91	304.2>107.1*，304.2>135.1	65	47，25
莱克多巴胺	莱克多巴胺-D₃	8.24	302.1>163.9*，302.1>107.0	65	23，56
克仑潘特	马布特罗-D₉	11.51	291.1>202.8*，291.1>273.1	65	24，15
氯丙那林	氯丙那林-D₇	8.90	214.1>154.1*，214.1>195.9	65	27，18
福莫特罗	莱克多巴胺-D₃	10.13	345.1>148.9*，345.1>326.8	65	27，19
妥布特罗	妥布特罗-D₉	10.94	228.1>153.8*，228.1>172.2	65	22，16
沙丁胺醇	沙丁胺醇-D₃	3.57	240.1>148.2*，240.1>222.1	65	27，15
班布特罗	班布特罗-D₉	11.69	368.2>294.1*，368.2>312.1	65	27，21
马布特罗	马布特罗-D₉	11.28	311.0>237.1*，311.0>217.1	65	24，37
西布特罗	西布特罗-D₉	4.38	234.2>159.8*，234.2>143.2	65	21，35
马布特罗-D₉		11.22	320.2>238.1	65	26
班布特罗-D₉		11.66	377.2>295.2	65	31
妥布特罗-D₉		10.87	237.2>155.1	65	21
氯丙那林-D₇		8.84	221.1>155.1	65	26
喷布特罗-D₉		14.47	301.2>237.1	65	26
特布他林-D₉		3.38	235.3>153.2	65	25
溴布特罗-D₉		10.99	376.1>294.0	65	29
莱克多巴胺-D₃		8.08	305.2>167.2	65	22
苯乙醇胺A-D₃		12.71	348.2>153.3	65	33
克伦特罗-D₉		9.38	286.2>204.2	65	24
沙丁胺醇-D₃		3.53	243.2>151.2	65	28
西布特罗-D₉		4.31	243.2>161.1	65	15
注：*为定量离子对。

下载: 导出CSV

表 2 17种β-受体激动剂线性范围、回归方程、相关系数、检出限及定量限

Table 2. Linear equation, correlation coefficient, LOD, LOQ of 17 β-receptor agonists

组分名称	线性范围（μg/L）	回归方程	相关系数	检出限（μg/kg）	定量限（μg/kg）
克仑潘特	0～20	y=0.0919x+−2.12e−013	r=0.9957	0.07	0.22
羟甲基克伦特罗	0～20	y=0.0435x+−1.16e−013	r=0.9984	0.09	0.31
马喷特罗	0～20	y=0.15x+1.45e−014	r=0.9991	0.04	0.12
喷布特罗	0～20	y=0.172x+−3.34e−014	r=0.9996	0.02	0.08
克伦特罗	0～20	y=0.0753x+3.28e−014	r=0.9999	0.08	0.25
特布他林	0～20	y=0.182x+1.5e−013	r=0.9990	0.05	0.16
溴布特罗	0～20	y=0.123x+−2.23e−013	r=0.9986	0.06	0.20
苯乙醇胺A	0～20	y=0.101x+−1.74e−014	r=0.9993	0.04	0.12
非诺特罗	0～20	y=0.273x+1.16e−012	r=0.9987	0.04	0.12
莱克多巴胺	0～20	y=0.199x+3.65e−013	r=0.9997	0.05	0.16
氯丙那林	0～20	y=0.105x+−3.54e−014	r=0.9998	0.04	0.14
福莫特罗	0～20	y=0.32x+7.85e−013	r=0.9995	0.03	0.09
妥布特罗	0～20	y=0.2x+7.87e−013	r=0.9981	0.05	0.15
沙丁胺醇	0～20	y=0.117x+−2.95e−013	r=0.9989	0.05	0.15
班布特罗	0～20	y=0.236x+−2.49e−013	r=0.9975	0.01	0.05
马布特罗	0～20	y=0.162x+4.56e−013	r=0.9980	0.03	0.11
西布特罗	0～20	y=0.164x+−6.21e−013	r=0.9982	0.08	0.28

下载: 导出CSV

表 3 17种β-受体激动剂回收率及精密度（n=6）

Table 3. Recoveries and RSD of 17 β-receptor agonists (n=6)

组分名称	加标水平（μg/kg）	猪肉		牛肉		羊肉
组分名称	加标水平（μg/kg）	回收率（%）	RSD（%）	回收率（%）	RSD（%）	回收率（%）	RSD（%）
克仑潘特	0.5	88.2	5.9	88.6	4.9	88.5	5.8
	2	88.6	4.9	88.9	3.3	87.8	3.1
	5	90.2	5.2	89.4	5.8	88.9	4.7
羟甲基克伦特罗	0.5	96.2	6.7	94.2	6.0	93.3	5.4
	2	94.3	5.4	93.7	7.7	93.5	4.5
	5	96.8	9.8	96.1	8.8	99.5	1.5
马喷特罗	0.5	92.9	1.2	95.7	7.8	91.2	1.7
	2	92.3	4.6	90.8	5.1	98.5	4.2
	5	89.1	3.2	91.0	3.2	94.7	2.4
喷布特罗	0.5	100.0	4.5	102.5	6.7	102.9	3.3
	2	102.9	4.8	103.7	4.4	108.0	1.4
	5	104.7	2.5	105.3	1.7	108.2	4.6
克伦特罗	0.5	99.8	3.1	95.4	2.8	103.3	6.4
	2	102.0	3.4	101.9	8.0	100.8	4.9
	5	100.6	1.7	99.8	4.0	98.3	4.2
特布他林	0.5	100.3	2.5	96.8	8.3	99.0	5.4
	2	101.1	2.5	91.4	4.8	97.8	9.3
	5	97.9	3.0	99.2	4.6	99.6	2.4
溴布特罗	0.5	100.8	1.0	106.5	3.3	106.9	3.6
	2	109.3	2.9	111.8	3.6	108.6	4.1
	5	107.0	2.9	110.8	5.7	103.4	4.6
苯乙醇胺A	0.5	101.9	7.2	101.4	8.3	105.5	6.7
	2	101.1	4.1	101.5	4.6	99.3	5.5
	5	100.3	3.9	103.0	8.0	101.8	1.5
非诺特罗	0.5	84.1	3.6	81.7	1.5	86.5	3.1
	2	83.9	2.4	84.8	4.2	92.2	6.7
	5	83.1	3.6	86.7	2.2	90.3	10.3
莱克多巴胺	0.5	91.0	1.6	93.1	4.6	95.5	7.6
	2	98.1	4.4	100.3	3.5	93.9	6.3
	5	100.4	3.5	100.1	4.5	97.3	6.6
氯丙那林	0.5	105.3	4.3	94.7	7.3	98.9	6.6
	2	101.9	2.8	103.7	3.9	99.5	1.4
	5	101.9	3.7	104.6	4.4	103.1	3.3
福莫特罗	0.5	96.3	2.7	99.3	6.6	99.6	5.5
	2	92.5	4.6	99.9	5.0	92.4	7.8
	5	93.8	6.0	105.0	1.0	98.5	2.7
妥布特罗	0.5	93.9	4.2	96.3	10.2	92.7	3.3
	2	92.2	4.3	91.8	4.4	92.5	4.7
	5	95.3	3.6	99.3	7.5	97.1	6.1
沙丁胺醇	0.5	102.3	4.2	90.9	2.7	94.3	3.7
	2	100.9	1.9	93.4	2.3	94.4	2.9
	5	99.3	0.8	105.0	4.4	102.9	5.2
班布特罗	0.5	100.4	1.1	100.8	4.1	100.7	1.5
	2	98.7	1.8	101.1	5.0	99.9	5.7
	5	102.3	6.0	100.7	6.7	98.5	7.0
马布特罗	0.5	98.3	0.8	99.8	4.8	106.6	2.9
	2	98.2	2.2	99.9	1.1	111.6	1.0
	5	105.8	1.8	101.5	5.6	104.9	1.6
西布特罗	0.5	100.6	5.8	99.2	6.6	106.4	2.6
	2	100.6	4.1	98.4	7.7	99.9	2.7
	5	97.0	6.5	103.3	8.2	111.2	1.4

下载: 导出CSV

参考文献(31)

[1]	XING G, YI C, DOU P Y, et al. Recent progress in the development of β₂ adrenergic receptor agonists: A patent review (2015-2020)[J]. Expert Opinion on Therapeutic Patents,2021,31(1/6):1354−3776.
[2]	HAQUE R, HAKIM A, MOODLEY T, et al. Inhaled long-acting β₂ agonists enhance glucocorticoid receptor nuclear translocation and efficacy in sputum macrophages in COPD[J]. The Journal of Allergy and Clinical Immunology,2013,132(5):1166−1173. doi: 10.1016/j.jaci.2013.07.038
[3]	陈清平, 韩峰, 汪洋, 等. 食源性动物组织中β-受体激动剂研究进展[J]. 食品安全质量检测学报,2019,10(2):385−393. [CHEN Qingping, HAN Feng, WANG Yang, et al. Research progress on β-agonists in food-borne animal tissues[J]. Journal of Food Safety and Quality,2019,10(2):385−393. CHEN Qingping, HAN Feng, WANG Yang, et al. Research progress on β-agonists in food-borne animal tissues [J]. Journal of Food Safety and Quality, 2019, 10(2): 385-393.
[4]	孙媛, 马晶, 马惠琴, 等. 猪肉、牛肉中5种β-受体激动剂残留的LC-MS/MS分析方法的建立[J]. 宁夏农林科技,2020,61(11):49−51, 54. [SUN Yuan, MA Jing, MA Huiqin, et al. LC-MS/MS method establishment for five kinds of β-receptor agonists residues in pork and beef[J]. Ningxia Journal of Agriculture and Forestry Science and Technology,2020,61(11):49−51, 54. SUN Yuan, MA Jing, MA huiqin, et al. LC-MS/MS method establishment for five kinds of β-receptor agonists residues in pork and beef [J]. Ningxia Journal of Agriculture and Forestry Science and Technology, 2020, 61(11): 49-51, 54.
[5]	张楠, 钱滢文, 吴丽华, 等. 基于离子液体柱前荧光衍生高效液相色谱法测定动物性食品中7 种β-受体激动剂[J]. 新宝登录入口(中国)有限公司,2021,42(23):296−302. [ZHANG Nan, QIAN Yingwen, WU Lihua, et al. Detection of 7 β-agonists in animal-derived food based on ionic liquid and pre-column fluorescence derivatization-high performance liquid chromatography[J]. Science and Technology of Food Industry,2021,42(23):296−302. ZHANG Nan, QIAN Yingwen, WU Lihua, et al. Detection of 7 β-agonists in animal-derived food based on ionic liquid and pre-column fluorescence derivatization-high performance liquid chromatography [J]. Science and Technology of Food Industry, 2021, 42(23): 296-302.
[6]	PROTTI M, SBERNA PM, SARDELLA R, et al. VAMS and StAGE as innovative tools for the enantioselective determination of clenbuterol in urine by LC-MS/MS[J]. Journal of Pharmaceutical and Biomedical Analysis,2021,195:113873. doi: 10.1016/j.jpba.2020.113873
[7]	LIU Rong, TANG Xiaoling, XIONG Renping, et al. Simultaneous determination of fourteen β₂-agonist enantiomers in food animal muscles by liquid chromatography coupled with tandem mass spectrometry[J]. Journal of Chromatography B,2022,1193:123169. doi: 10.1016/j.jchromb.2022.123169
[8]	MASTRIANNI K R, METAVARAYUTH K, BREWER W E, et al. Analysis of 10 β-agonists in pork meat using automated dispersive pipette extraction and LC-MS/MS[J]. Journal of Chromatography B,2018,1084:64−68. doi: 10.1016/j.jchromb.2018.03.026
[9]	HOFF RB, MOLOGNONI L, DEOLINDO CTP, et al. Determination of 62 veterinary drugs in feedingstuffs by novel pressurized liquid extraction methods and LC-MS/MS[J]. Journal of Chromatography B,2020,1152:122232. doi: 10.1016/j.jchromb.2020.122232
[10]	邓艳芹, 汪坤乾, 廖金兰, 等. 动物源样品中β-受体激动剂的测定方法[J]. 咸阳师范学院学报,2022,37(4):25−29. [DENG Yanqin, WANG Kunqian, LIAO Jinlan, et al. A method for β-adrenoceptor agonists residues detection in animal-derived samples[J]. Journal of Xianyang Normal University,2022,37(4):25−29. doi: 10.3969/j.issn.1672-2914.2022.04.007 DENG Yanqin, WANG Kunqian, LIAO Jinlan, et al. A method for β-adrenoceptor agonists residues detection in animal-derived samples [J]. Journal of Xianyang Normal University, 2022, 37(4): 25-29. doi: 10.3969/j.issn.1672-2914.2022.04.007
[11]	岳韩笑, 雷雯, 杜晓宁, 等. 同位素稀释-气相色谱-串联质谱法测定猪肉中残留的4种β-受体激动剂[J]. 质谱学报,2018,39(1):61−68. [YUE Hanxiao, LEI Wen, DU Xiaoning, et al. Multi-residue analysis of 4 β-agonists in pork using isotope dilution gas chromatography-tandem mass spectrometry[J]. Journal of Chinese Mass Spectrometry Society,2018,39(1):61−68. YUE Hanxiao, LEI Wen, DU Xiaoning, et al. Multi-residue analysis of 4 β-agonists in pork using isotope dilution gas chromatography-tandem mass spectrometry [J]. Journal of Chinese Mass Spectrometry Society, 2018, 39(1): 61-68.
[12]	刘淼苗, 刘啊敏, 陈尔净, 等. 基于二聚人工抗原ELISA检测多残留β₂-受体激动剂[J]. 中国计量大学学报,2022,33(1):27−33. [LIU Miaomiao, LIU Amin, CHEN Erjing, et al. Eetection of β₂-agonists multi-residual by ELISA based on dimeric artificial antigen[J]. Journal of China University of Metrology,2022,33(1):27−33. LIU Miaomiao, LIU Amin, CHEN Erjing, et al. Eetection of β₂-agonists multi-residual by ELISA based on dimeric artificial antigen [J]. Journal of China University of Metrology, 2022, 33(1): 27-33.
[13]	李延山, 刘庆伟, 卞大伟, 等. ELISA检测猪尿中β-受体激动剂类药物影响因素研究[J]. 动物医学进展,2018,39(8):78−82. [LI Yanshan, LIU Qingwei, BIAN Dawei, et al. Study on influencing factors for detecting β-receptor agonists in porcine urine by ELISA[J]. Progress in Veterinary Medicine,2018,39(8):78−82. LI Yanshan, LIU Qingwei, BIAN Dawei, et al. Study on influencing factors for detecting β-receptor agonists in porcine urine by ELISA [J]. Progress in Veterinary Medicine, 2018, 39(8): 78-82.
[14]	WANG Weiyu, ZHANG Yulian, WANG Jinyan, et al. Determination of beta-agonists in pig feed, pig urine and pig liver using capillary electrophoresis with electrochemical detection[J]. Meat Science,2010,85(2):302−305. doi: 10.1016/j.meatsci.2010.01.018
[15]	周迎春, 华向美, 李单单. β-受体激动剂的性质及其残留量测定时的关键点[J]. 肉类工业,2021,483(7):34−39. [ZHOU Yingchun, HUA Xiangmei, LI Dandan. The properties of β-agonists and the key points in determination of residue[J]. Meat Industry,2021,483(7):34−39. ZHOU Yingchun, HUA Xiangmei, LI Dandan. The properties of β-agonists and the key points in determination of residue [J]. Meat Industry, 2021, 483(7): 34-39.
[16]	WANG G, ZHAO J, PENG T, et al. Matrix effects in the determination of β-receptor agonists in animal-derived foodstuffs by ultra-performance liquid chromatography tandem mass spectrometry with immunoaffinity solid-phase extraction[J]. Journal of separation science,2013,36(4):796−802. doi: 10.1002/jssc.201200661
[17]	崔利辉, 李璐琦, 李国秀, 等. 超高效液相色谱-串联质谱法测定牛肉中9种β-受体激动剂[J]. 食品安全质量检测学报,2021,12(12):4858−4866. [CUI Lihui, LI Luqi, LI Guoxiu, et al. Determination of 9 kinds of β-agonists in beef by ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality,2021,12(12):4858−4866. CUI Lihui, LI Luqi, LI Guoxiu, et al. Determination of 9 kinds of β-agonists in beef by ultra performance liquid chromatography-tandem mass spectrometry [J]. Journal of Food Safety and Quality, 2021, 12(12): 4858-4866.
[18]	肖勇, 万伟杰, 邬磊, 等. QuEChERS-高效液相色谱-串联质谱法测定动物源性产品中9种β-受体激动剂残留[J]. 江西农业学报,2021,33(2):102−108. [XIAO Yong, WAN Weijie, WU Lei, et al. Determination of 9 β-agonist residues in animal products by QuEChERS-high performance liquid chromatography-tandem mass spectrometry[J]. Acta Agriculturae Jiangxi,2021,33(2):102−108. XIAO Yong, WAN Weijie, WU Lei, et al. Determination of 9 β-agonist residues in animal products by QuEChERS-high performance liquid chromatography-tandem mass spectrometry [J]. Acta Agriculturae Jiangxi, 2021, 33(2): 102-108.
[19]	XIONG Lin, GAO Yaqin, LI Weihong, et al. Simple and sensitive monitoring of β₂-agonist residues in meat by liquid chromatography-tandem mass spectrometry using a QuEChERS with preconcentration as the sample treatment[J]. Meat Science,2015,7(105):96−107.
[20]	胡雪郢, 薛丰. 脂质特异基质分散萃取超高效液相色谱-串联质谱法测定肉制品中4种兽药残留[J]. 化学分析计量,2021,30(7):17−22. [HU Xueying, XUE Feng. Determination of 4 agonist residues in meat by lipid-specific matrix dispersed purification combined with ultra performance liquid chromatography tandem mass spectrometry[J]. Chemical Analysis and Meterage,2021,30(7):17−22. HU Xueying, XUE Feng. Determination of 4 agonist residues in meat by lipid-specific matrix dispersed purification combined with ultra performance liquid chromatography tandem mass spectrometry [J]. Chemical Analysis and Meterage, 2021, 30(7): 17-22.
[21]	刘学芝, 赵英莲, 马跃, 等. 超高效液相色谱-串联质谱法测定猪肉、鸡蛋、牛奶中9种食源性兴奋剂类药物残留[J]. 色谱,2022,40(2):148−155. [LIU Xuezhi, ZHAO Yinglian, MA Yue, et al. Determination of nine food-borne stimulant drug residues in pork, egg, and milk by ultra-performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography,2022,40(2):148−155. doi: 10.3724/SP.J.1123.2021.04005 LIU Xuezhi, ZHAO Yinglian, MA Yue, et al. Determination of nine food-borne stimulant drug residues in pork, egg, and milk by ultra-performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2022, 40(2): 148-155. doi: 10.3724/SP.J.1123.2021.04005
[22]	李道霞, 黄丽娟, 辜慧, 等. HPLC-MS/MS法检测动物源性食品中喹诺酮类药物的基质效应及其补偿措施研究[J]. 中国食品卫生杂志,2022,34(4):693−699. [LI Daoxia, HUANG Lijuan, GU Hui, et al. Matrix effects and compensation measures of quinolones detection in animal derived food by HPLC-MS/MS[J]. Chinese Journal of Food Hygine,2022,34(4):693−699. LI Daoxia, HUANG Lijuan, GU Hui, et al. Matrix effects and compensation measures of quinolones detection in animal derived food by HPLC-MS/MS [J]. Chinese Journal of Food Hygine, 2022, 34(4): 693-699.
[23]	王丹, 马淑青, 吴芳平, 等. PXC 和EMR-Lipid QuEChERs 技术在动物源性食品中多种β-受体激动剂残留检测的前处理效果对比[J]. 中国卫生工程学,2021,20(1):30−33. [WANG Dan, MA Shuqing, WU Fangping, et al. The comparison of the pretreatment effects of PXC and EMR-Lipid QuEChERs technology in the detection of multiple β-receptor agonist residues in animal-derived food[J]. Chinese Journal of Public Health Engineering,2021,20(1):30−33. WANG Dan, MA Shuqing, WU Fangping, et al. The comparison of the pretreatment effects of PXC and EMR-Lipid QuEChERs technology in the detection of multiple β-receptor agonist residues in animal-derived food [J]. Chinese Journal of Public Health Engineering, 2021, 20(1): 30-33.
[24]	李磊, 李海畅, 高婧, 等. QuEChERS EMR-Lipid-LC/MS/MS测定8种β-受体激动剂[J]. 食品研究与开发,2016,37(9):178−182. [LI Lei, LI Haichang, GAO Jing, et al. Determination of 8 β-agonists by QuEChERS EMR-Lipid-LC/MS/MS[J]. Food Research And Development,2016,37(9):178−182. LI Lei, LI Haichang, GAO Jing, et al. Determination of 8 β-agonists by QuEChERS EMR-Lipid-LC/MS/MS [J]. Food Research And Development, 2016, 37(9): 178-182.
[25]	YU Yang, YAO Changliang, GUO Dean. Insight into chemical basis of traditional Chinese medicine based on the state-of-the-art techniques of liquid chromatography-mass spectrometry[J]. Acta Pharmaceutica Sinica B,2021,11(6):1467−1492.
[26]	丁宁, 董亚蕾, 张秋. UHPLC-MS/MS法同时测定祛痘类化妆品中63种激素类药物[J]. 日用化学工业,2022,45(8):11−39. [DING Ning, DONG Yalei, ZHANG Qiu. Simultaneous determination of 63 hormone drugs in anti-acne cosmetics by UHPLC-MS/MS[J]. China Surfactant Detergent & Cosmetics,2022,45(8):11−39. DING Ning, DONG Yalei, ZHANG Qiu. Simultaneous determination of 63 hormone drugs in anti-acne cosmetics by UHPLC-MS/MS [J]. China Surfactant Detergent & Cosmetics, 2022, 45(8): 11-39.
[27]	中华人民共和国国家质量监督检验检疫局, 中国国家标准化管理委员会. GB/T 22286-2008 动物源性食品中多种β-受体激动剂残留量的测定液相色谱串联质谱法[S]. 北京: 中国标准出版社, 2008 General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, China National Standardization Administration Committee. GB/T 22286-2008 Determination of multiple β-receptor agonist residues in animal derived food high performance liquid chromatography-tandem mass spectrometry [S]. Beijing: Standards Press of China, 2008.
[28]	中华人民共和国国家质量监督检验检疫局, 中国国家标准化管理委员会. GB/T 21313-2007 动物源性食品中β-受体激动剂残留检测方法液相色谱-质谱/质谱法[S]. 北京: 中国标准出版社, 2007 General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, China National Standardization Administration Committee. GB/T 21313-2007 Determination of β-receptor agonist residues in animal derived food high performance liquid chromatography-tandem mass spectrometry [S]. Beijing: Standards Press of China, 2007.
[29]	俞晓兰, 夏宝林, 张维益, 等. 超高效液相色谱-串联质谱法测定鸡肉中5种β-受体激动剂残留量[J]. 食品安全质量检测学报,2018,9(16):4320−4325. [YU Xxiaolian, XIAO Baolin, ZHANG Weiyi, et al. Determination of 5 kinds of β-agonist residues in chicken by ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality,2018,9(16):4320−4325. YU Xxiaolian, XIAO Baolin, ZHANG Weiyi, et al. Determination of 5 kinds of β-agonist residues in chicken by ultra performance liquid chromatography-tandem mass spectrometry [J]. Journal of Food Safety and Quality, 2018, 9(16): 4320-4325.
[30]	王守英, 司文帅, 杨海锋, 等. 高效液相色谱-串联质谱法测定动物尿液中23种β-受体激动剂[J]. 食品安全质量检测学报,2021,12(14):5620−5628. [WANG Shouying, SI Wenshuai, YANG Haifeng, et al. Determination of 23 kinds of β-receptor agonists in animal urine by high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality,2021,12(14):5620−5628. WANG Shouying, SI Wenshuai, YANG Haifeng, et al. Determination of 23 kinds of β-receptor agonists in animal urine by high performance liquid chromatography-tandem mass spectrometry [J]. Journal of Food Safety and Quality, 2021, 12(14): 5620-5628.
[31]	谢瑜杰, 李铁梅, 牛相涛, 等. 一步式QuEChERS方法结合高效液相色谱-串联质谱法测定牛肉中25 种磺胺类药物残留[J]. 新宝登录入口(中国)有限公司,2021,42(20):268−278. [XIE Yujie, LI Tiemei, NIU Xiangtao, et al. Determination of sulfonamides in beef by one step QuEChERS method combined with high performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry,2021,42(20):268−278. XIE Yujie, LI Tiemei, NIU Xiangtao, et al. Determination of sulfonamides in beef by one step QuEChERS method combined with high performance liquid chromatography-tandem mass spectrometry [J]. Science and Technology of Food Industry, 2021, 42(20): 268-278.